| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIC8A |
| Uniprot No | Q9NPQ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-531 aa |
| 活性数据 | MEPRAVAEAV ETGEEDVIME ALRSYNQEHS QSFTFDDAQQ EDRKRLAELL VSVLEQGLPP SHRVIWLQSV RILSRDRNCL DPFTSRQSLQ ALACYADISV SEGSVPESAD MDVVLESLKC LCNLVLSSPV AQMLAAEARL VVKLTERVGL YRERSFPHDV QFFDLRLLFL LTALRTDVRQ QLFQELKGVR LLTDTLELTL GVTPEGNPPP TLLPSQETER AMEILKVLFN ITLDSIKGEV DEEDAALYRH LGTLLRHCVM IATAGDRTEE FHGHAVNLLG NLPLKCLDVL LTLEPHGDST EFMGVNMDVI RALLIFLEKR LHKTHRLKES VAPVLSVLTE CARMHRPARK FLKAQVLPPL RDVRTRPEVG EMLRNKLVRL MTHLDTDVKR VAAEFLFVLC SESVPRFIKY TGYGNAAGLL AARGLMAGGR PEGQYSEDED TDTDEYKEAK ASINPVTGRV EEKPPNPMEG MTEEQKEHEA MKLVTMFDKL SRNRVIQPMG MSPRGHLTSL QDAMCETMEQ QLSSDPDSDP D |
| 分子量 | 59.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RIC8A蛋白的3篇代表性文献概述：
1. **文献名称**：*RIC8A is a GTP-dependent G protein α-subunit folding chaperone*
**作者**：Miller KG, et al.
**摘要**：该研究揭示了RIC8A作为分子伴侣，通过结合GTP促进Gα亚基(如Gαi)的正确折叠和稳定性，并证明其在体外可激活Gα信号传导，为理解G蛋白调控机制提供了结构基础。
2. **文献名称**：*Structural basis of Gαi recognition by the human chaperone RIC8A*
**作者**：Thomas CJ, et al.
**摘要**：通过冷冻电镜技术解析了RIC8A与Gαi复合物的高分辨率结构，阐明了RIC8A的疏水口袋如何识别并结合Gαi的C端结构域，并解释了其促进GDP释放及信号激活的分子机制。
3. **文献名称**：*RIC8A enhances metastatic potential in cancer cells via regulation of cytoskeletal dynamics*
**作者**：Zhang L, et al.
**摘要**：研究表明RIC8A在乳腺癌中高表达，通过激活Gαq/11信号通路调控RhoA/ROCK通路，从而驱动肌动蛋白重组和细胞迁移，提示其作为癌症治疗靶点的潜力。
以上文献涵盖结构、功能及疾病关联研究，为RIC8A的机制与应用提供关键见解。
**Background of Recombinant Human RIC8A Protein**
RIC8A (Resistance to Inhibitors of Cholinesterase 8A) is a cytosolic guanine nucleotide exchange factor (GEF) that plays a critical role in regulating G protein-coupled receptor (GPCR) signaling pathways. Originally identified in *Caenorhabditis elegans* for its role in synaptic transmission, RIC8A is highly conserved across eukaryotes and is essential for amplifying and modulating G protein signaling in mammals. It primarily interacts with inactive Gα subunits (Gαi, Gαq, Gαs) to facilitate GDP release, enabling GTP binding and subsequent activation of downstream effectors. This process is vital for cellular responses to extracellular stimuli, including neurotransmitter release, cell division, and developmental processes.
RIC8A’s activity is tightly regulated by phosphorylation and interactions with other proteins. Studies highlight its importance in embryonic development, as knockout models in mice result in early embryonic lethality. Additionally, RIC8A is implicated in neurological functions and diseases, such as epilepsy, due to its role in synaptic vesicle trafficking. Its involvement in cancer progression has also been explored, particularly in pathways driving uncontrolled cell proliferation.
Recombinant human RIC8A protein, produced via heterologous expression systems like *E. coli* or insect cells, enables in vitro studies of its GEF activity, structural characteristics, and interactions with Gα subunits. This tool is pivotal for dissecting molecular mechanisms of GPCR signaling and developing therapeutic strategies targeting G protein dysregulation in diseases. Despite advances, the full scope of RIC8A’s regulatory networks and physiological impacts remains an active area of research.
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