| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHOXF1 |
| Uniprot No | Q8NHV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-184 aa |
| 活性数据 | MARSLVHDTV FYCLSVYQVK ISPTPQLGAA SSAEGHVGQG APGLMGNMNP EGGVNHENGM NRDGGMIPEG GGGNQEPRQQ PQPPPEEPAQ AAMEGPQPEN MQPRTRRTKF TLLQVEELES VFRHTQYPDV PTRRELAENL GVTEDKVRVW FKNKRARCRR HQRELMLANE LRADPDDCVY IVVD |
| 分子量 | 20.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RHOXF1蛋白的3篇参考文献概括(基于领域研究主题综合整理，非真实存在的具体文献)：
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1. **标题**：*RHOXF1 regulates human spermatogenesis through transcriptional activation of testis-specific genes*
**作者**：A. Rossi et al.
**摘要**：研究发现RHOXF1在人类睾丸生精细胞中特异性高表达，通过调控下游靶基因(如PRM1、TNP1)参与精子形成过程，敲除实验表明其缺失会导致精子形态异常和生育力下降。
2. **标题**：*Cancer-testis antigen RHOXF1 promotes tumorigenesis via Wnt/β-catenin signaling in colorectal cancer*
**作者**：M. Tanaka et al.
**摘要**：揭示RHOXF1在结直肠癌等多种肿瘤中异常激活，通过结合β-catenin增强Wnt信号通路活性，促进肿瘤细胞增殖和转移，提示其作为潜在治疗靶点。
3. **标题**：*RHOXF1 mutations associated with X-linked intellectual disability and impaired neuronal differentiation*
**作者**：S. Kimmins et al.
**摘要**：首次报道RHOXF1基因突变与X染色体连锁智力障碍的相关性，功能实验证明突变体破坏RHOXF1的DNA结合能力，导致皮层神经元分化障碍和突触功能异常。
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*注：以上为模拟生成的文献摘要，反映RHOXF1在生殖发育、癌症、神经疾病中的研究热点。实际文献需通过PubMed等数据库检索确认。*
**Background of Recombinant Human RHOXF1 Protein**
RHOXF1 (Rhox Homeobox Family Member 1), also known as PEPP2. is a member of the Rhox gene cluster located on the X chromosome. This cluster encodes homeodomain-containing transcription factors preferentially expressed in reproductive tissues. RHOXF1 is specifically localized to the nucleus and plays critical roles in regulating genes involved in gametogenesis, embryogenesis, and reproductive system development. Structurally, it contains a conserved N-terminal homeodomain responsible for DNA binding and a C-terminal region that may mediate transcriptional activation or repression.
RHOXF1 is predominantly expressed in the testis, particularly in germ cells during spermatogenesis, suggesting its importance in male fertility. Studies indicate its involvement in modulating cell proliferation, differentiation, and apoptosis within the male reproductive tract. Additionally, RHOXF1 has been implicated in regulating placental development and maternal-fetal interactions. Dysregulation of RHOXF1 is linked to reproductive disorders, such as spermatogenic failure, and certain cancers, including prostate and testicular germ cell tumors, where it may act as an oncogene or tumor suppressor depending on context.
Recombinant RHOXF1 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables functional studies, including DNA-binding assays, transcriptional regulation analyses, and interaction partner identification. Its application aids in elucidating molecular mechanisms in reproduction and disease, offering potential diagnostic or therapeutic avenues.
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