| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHPN1 |
| Uniprot No | Q8TCX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-695 aa |
| 活性数据 | MILEERPDGA GAGEESPRLQ GCDSLTQIQC GQLQSRRAQI HQQIDKELQM RTGAENLYRA TSNNRVRETV ALELSYVNSN LQLLKEELEE LSGGVDPGRH GSEAVTVPMI PLGLKETKEL DWSTPLKELI SVHFGEDGAS YEAEIRELEA LRQAMRTPSR NESGLELLTA YYNQLCFLDA RFLTPARSLG LFFHWYDSLT GVPAQQRALA FEKGSVLFNI GALHTQIGAR QDRSCTEGAR RAMEAFQRAA GAFSLLRENF SHAPSPDMSA ASLCALEQLM MAQAQECVFE GLSPPASMAP QDCLAQLRLA QEAAQVAAEY RLVHRTMAQP PVHDYVPVSW TALVHVKAEY FRSLAHYHVA MALCDGSREC PPHLPMVLPR PPRAGSQPLC PPAATEGELP THEQVFLQPP TSSKPRGPVL PQELEERRQL GKAHLKRAIL GQEEALRLHA LCRVLREVDL LRAVISQTLQ RSLAKYAELD REDDFCEAAE APDIQPKTHQ KPEARMPRLS QGKGPDIFHR LGPLSVFSAK NRWRLVGPVH LTRGEGGFGL TLRGDSPVLI AAVIPGSQAA AAGLKEGDYI VSVNGQPCRW WRHAEVVTEL KAAGEAGASL QVVSLLPSSR LPSLGDRRPV LLGPRGLLRS QREHGCKTPA STWASPRPLL NWSRKAQQGK TGGCPQPCAP VKPAPPSSLK HPGWP |
| 分子量 | 73.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RHPN1蛋白的3篇示例参考文献(注：以下内容为假设性示例，真实文献需补充具体信息)：
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1. **文献名称**: *RHPN1 drives ovarian cancer metastasis via RhoA/ROCK signaling*
**作者**: Wang, X. et al.
**摘要**: 研究揭示了重组RHPN1蛋白通过激活RhoA/ROCK通路增强卵巢癌细胞侵袭能力，实验证实其过表达促进体内外转移。
2. **文献名称**: *RHPN1 interacts with Wnt/β-catenin to regulate melanoma progression*
**作者**: Zhang, L. et al.
**摘要**: 报道了重组RHPN1蛋白与β-catenin结合，促进黑色素瘤细胞增殖和迁移，提示其作为治疗靶点的潜力。
3. **文献名称**: *Extracellular RHPN1 as a diagnostic biomarker in pancreatic cancer*
**作者**: Liu, Y. et al.
**摘要**: 通过重组RHPN1蛋白检测发现其在胰腺癌患者血清中高表达，可作为早期诊断标志物。
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提示：实际文献需通过**PubMed/Google Scholar**查询关键词“RHPN1 recombinant protein”或“RHPN1 function”，并补充期刊名称、年份等完整信息。是否需要进一步协助检索真实文献?
**Background of Recombinant Human RHPN1 Protein**
The recombinant human RHPN1 (Rho GTPase-binding protein 1) protein is a member of the Rhophilin family, encoded by the *RHPN1* gene. It primarily interacts with Rho GTPases, key regulators of cytoskeletal dynamics, cell adhesion, and motility. Structurally, RHPN1 contains a conserved N-terminal pleckstrin homology (PH) domain and a C-terminal coiled-coil domain, enabling its role in signal transduction and protein-protein interactions.
RHPN1 is implicated in cellular processes such as actin cytoskeleton remodeling, cell polarization, and extracellular matrix organization. Dysregulation of RHPN1 has been linked to cancer progression, particularly in metastasis, due to its influence on RhoA/Rho kinase (ROCK) pathways. Elevated RHPN1 expression is observed in cancers like breast and gastric carcinoma, correlating with poor prognosis. Additionally, it may contribute to neurological disorders by affecting neuronal morphology and synaptic plasticity.
Recombinant RHPN1 proteins, typically produced in *E. coli* or mammalian expression systems (e.g., HEK293 cells), retain functional domains for *in vitro* studies. These proteins are used to investigate molecular mechanisms, screen therapeutic inhibitors, or develop diagnostic biomarkers. Their purity and activity are validated via SDS-PAGE, Western blot, and GTPase-binding assays. As a potential therapeutic target, RHPN1 remains under active exploration in oncology and neurology research.
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