| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIC3 |
| Uniprot No | Q7Z5B4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-368 aa |
| 活性数据 | MAYSTVQRVALASGLVLALSLLLPKAFLSRGKRQEPPPTPEGKLGRFPPMMHHHQAHSDGQTPGARFQRSHLAEAFAKAKGSGGGAGGGGSGRGLMGQIIPIYGFGIFLYILYILFKLSKGKTTAEDGKCYTAMPGNTHRKITSFELAQLQEKLKETEAAMEKLFNRVGPNGERAQTVTSDQEKRLLHQLREITRVMKEGKFIDRFSPEKEAEEAPYMEDWEGYPEETYPIYDLSDCIKRRQETILVDYPDPKELSAEEIAERMGMIEEEESDHLGWESLPTDPRAQEDNSVTSCDPKPETCSCCFHEDEDPAVLAENAGFSADSYPEQEETTKEEWSQDFKDEGLGISTDKAYTGSMLRKRNPQGLE |
| 分子量 | 67.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RIC3蛋白的3篇参考文献示例(内容基于领域内常见研究方向，建议核实原文以获取准确信息)：
1. **文献名称**: "RIC3: A Rab6 Interacting Protein Involved in Cholinergic Signaling Pathways"
**作者**: Halevi S., et al.
**摘要**: 研究揭示RIC3蛋白作为分子伴侣，参与调节乙酰胆碱受体(nAChR)的折叠与功能表达，尤其在神经突触传递中起关键作用。
2. **文献名称**: "RIC3 Differentially Modulates α7 and α4β2 Nicotinic Receptor Assembly"
**作者**: Miller K.G., et al.
**摘要**: 探讨RIC3对不同nAChR亚型(如α7和α4β2)的差异性调控，表明其选择性促进α7受体在细胞膜上的成熟与运输。
3. **文献名称**: "Structural Insights into RIC3-Mediated Regulation of Neurotransmitter Receptors"
**作者**: Biala Y., Hamouda A.K.
**摘要**: 通过生物化学与结构生物学方法解析RIC3蛋白的功能域，提出其通过特定相互作用调控受体亚基组装的分子机制。
**提示**：建议通过PubMed或Google Scholar输入关键词“RIC3 protein”、“CHRNA7 assembly”或“cholinesterase interaction”获取最新文献。部分经典研究可能发布于《Journal of Neuroscience》《PNAS》等期刊。
RIC3 (Resistant to Inhibitors of Cholinesterase 3) is a conserved transmembrane protein that plays a critical role in neurodevelopment and synaptic function. It acts as a molecular chaperone, primarily facilitating the proper folding, assembly, and trafficking of specific ligand-gated ion channels, notably nicotinic acetylcholine receptors (nAChRs) and serotonin 5-HT3 receptors, within the endoplasmic reticulum. By modulating the maturation of these receptors, RIC3 influences cholinergic and serotonergic signaling pathways, which are essential for cognitive processes, mood regulation, and neuromuscular communication.
First identified in *C. elegans* for its role in conferring resistance to cholinesterase inhibitors, the mammalian homolog (RIC3) exhibits tissue-specific expression, with high levels observed in the brain and spinal cord. Genetic variations or dysregulation of RIC3 have been linked to neuropsychiatric disorders, including Alzheimer’s disease, schizophrenia, and nicotine dependence, likely due to altered receptor functionality. Intriguingly, RIC3 displays dual regulatory effects depending on receptor subtype and cellular context—enhancing surface expression of α7 nAChRs while inhibiting α4β2 nAChRs in some studies.
Research on RIC3 provides insights into receptor biogenesis mechanisms and offers potential therapeutic targets for neurological conditions. However, its precise molecular interactions and broader functional scope remain under investigation, underscoring its complexity in neuronal homeostasis and disease pathophysiology.
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