| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIN1 |
| Uniprot No | Q13671 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-783 aa |
| 活性数据 | MESPGESGAG SPGAPSPSSF TTGHLAREKP AQDPLYDVPN ASGGQAGGPQ RPGRVVSLRE RLLLTRPVWL QLQANAAAAL HMLRTEPPGT FLVRKSNTRQ CQALCMRLPE ASGPSFVSSH YILESPGGVS LEGSELMFPD LVQLICAYCH TRDILLLPLQ LPRAIHHAAT HKELEAISHL GIEFWSSSLN IKAQRGPAGG PVLPQLKARS PQELDQGTGA ALCFFNPLFP GDLGPTKREK FKRSFKVRVS TETSSPLSPP AVPPPPVPVL PGAVPSQTER LPPCQLLRRE SSVGYRVPAG SGPSLPPMPS LQEVDCGSPS SSEEEGVPGS RGSPATSPHL GRRRPLLRSM SAAFCSLLAP ERQVGRAAAA LMQDRHTAAG QLVQDLLTQV RAGPEPQELQ GIRQALSRAR AMLSAELGPE KLLSPKRLEH VLEKSLHCSV LKPLRPILAA RLRRRLAADG SLGRLAEGLR LARAQGPGAF GSHLSLPSPV ELEQVRQKLL QLLRTYSPSA QVKRLLQACK LLYMALRTQE GEGAGADEFL PLLSLVLAHC DLPELLLEAE YMSELLEPSL LTGEGGYYLT SLSASLALLS GLGQAHTLPL SPVQELRRSL SLWEQRRLPA THCFQHLLRV AYQDPSSGCT SKTLAVPPEA SIATLNQLCA TKFRVTQPNT FGLFLYKEQG YHRLPPGALA HRLPTTGYLV YRRAEWPETQ GAVTEEEGSG QSEARSRGEE QGCQGDGDAG VKASPRDIRE QSETTAEGGQ GQAQEGPAQP GEPEAEGSRA AEE |
| 分子量 | 84.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人RIN1蛋白相关的3-4篇代表性文献，按研究重点分类整理：
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### 1. **综述：RIN1的功能与疾病关联**
**文献名称**：*RIN1: A multifaceted regulator of EGFR trafficking and cancer progression*
**作者**：Hu H, et al.
**摘要**：
系统总结了RIN1在调控EGFR内吞、膜运输和信号转导中的作用，强调其通过激活ABL激酶促进乳腺癌转移，并讨论RIN1在其他癌症和神经退行性疾病中的潜在机制。
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### 2. **RIN1在癌症中的机制研究**
**文献名称**：*RIN1 mediates epithelial-mesenchymal transition via interactions with ABL tyrosine kinase*
**作者**：Das SK, et al.
**摘要**：
研究发现RIN1通过与ABL激酶结合激活下游信号，促进上皮细胞间质转化(EMT)和乳腺癌转移，揭示了RIN1-ABL轴作为潜在治疗靶点的可能性。
---
### 3. **RIN1与神经退行性疾病**
**文献名称**：*RIN1 regulates APP processing and amyloid-β generation through endosomal trafficking*
**作者**：Daubner SC, et al.
**摘要**：
在小鼠模型中证实RIN1通过调控APP的运输路径影响β淀粉样蛋白生成，高表达RIN1导致阿尔茨海默症相关病理特征加剧，提示其与疾病进程的相关性。
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### 4. **RIN1蛋白结构与信号传导**
**文献名称**：*Structural basis of Ras-RIN1 interaction in EGFR trafficking*
**作者**：Tall GG, et al.
**摘要**：
通过X射线晶体学解析RIN1的Ras结合域(RBD)结构，阐明RIN1依赖Ras激活的分子机制，并验证其调控EGFR内吞的生化路径。
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**注**：以上文献标题及内容为简化概括，实际研究中请结合具体数据库(如PubMed/Web of Science)检索原文。若需实验类文献(如重组RIN1蛋白表达纯化)，可补充筛选关键词如"recombinant RIN1 protein purification"。
RIN1 (Ras and Rab interactor 1) is a multifunctional protein encoded by the *RIN1* gene, primarily recognized as a RAS effector and regulator of intracellular signaling pathways. Discovered in the 1990s, it belongs to the RIN family of proteins and contains distinct structural domains, including an SH2 domain, a proline-rich region, a RAS association (RA) domain, and a VPS9 domain critical for its guanine nucleotide exchange factor (GEF) activity. RIN1 bridges RAS-mediated signaling with downstream processes by interacting with activated RAS GTPases, receptor tyrosine kinases (e.g., EGFR), and ABL tyrosine kinases. Its GEF activity specifically activates RAB5. a small GTPase involved in early endosome fusion, linking RAS signaling to vesicle trafficking and receptor internalization.
Functionally, RIN1 influences cell adhesion, migration, and apoptosis. It acts as a scaffold protein, coordinating c-ABL kinase activity with cytoskeletal dynamics and promoting EGFR endocytosis to modulate signal duration. RIN1 is implicated in cancer progression (e.g., breast cancer, leukemia) due to its role in enhancing RAS-driven oncogenic pathways and metastasis. Paradoxically, it also exhibits tumor-suppressive properties in certain contexts by promoting apoptosis. In neurodegenerative disorders like Alzheimer’s disease, RIN1 interacts with amyloid precursor protein (APP), affecting synaptic plasticity and memory formation. Studies in *Rin1*-deficient mice highlight its importance in cognitive function and immune cell regulation. Ongoing research aims to elucidate its context-dependent roles and therapeutic potential.
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