| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RILPL1 |
| Uniprot No | Q5EBL4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-403 aa |
| 活性数据 | MEEERGSALA AESALEKNVA ELTVMDVYDI ASLVGHEFER VIDQHGCEAI ARLMPKVVRV LEILEVLVSR HHVAPELDEL RLELDRLRLE RMDRIEKERK HQKELELVED VWRGEAQDLL SQIAQLQEEN KQLMTNLSHK DVNFSEEEFQ KHEGMSERER QVMKKLKEVV DKQRDEIRAK DRELGLKNED VEALQQQQTR LMKINHDLRH RVTVVEAQGK ALIEQKVELE ADLQTKEQEM GSLRAELGKL RERLQGEHSQ NGEEEPETEP VGEESISDAE KVAMDLKDPN RPRFTLQELR DVLHERNELK SKVFLLQEEL AYYKSEEMEE ENRIPQPPPI AHPRTSPQPE SGIKRLFSFF SRDKKRLANT QRNVHIQESF GQWANTHRDD GYTEQGQEAL QHL |
| 分子量 | 47.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RILPL1蛋白的3篇参考文献(示例为假设性文献，仅供参考)：
1. **文献名称**：RILPL1调节纤毛生成与Rab8功能互作的分子机制
**作者**：M. Yamamoto等(2018)
**摘要**：研究发现RILPL1通过与Rab8 GTP酶结合，调控初级纤毛的形成。实验表明，RILPL1缺失导致纤毛组装障碍，提示其在细胞极性建立中的关键作用。
2. **文献名称**：RILPL1在帕金森病相关LRRK2信号通路中的作用
**作者**：H. Smith等(2019)
**摘要**：揭示LRRK2激酶通过磷酸化RILPL1的Thr部位，改变其与细胞骨架蛋白互作能力，可能参与神经退行性疾病中轴突运输异常。
3. **文献名称**：RILPL1介导的自噬体-溶酶体融合机制研究
**作者**：L. Chen等(2020)
**摘要**：RILPL1通过与Rab34和LC3蛋白互作，调控自噬体成熟。研究为癌症细胞内代谢紊乱提供了潜在治疗靶点。
*注：以上内容为模拟文献，建议通过PubMed/Google Scholar核实真实论文。*
Recombinant human RILPL1 (Rab-interacting lysosomal protein-like 1) is a cytosolic protein belonging to the RILP (Rab-interacting lysosome protein) family. It contains an N-terminal coiled-coil domain and a C-terminal Rab-binding domain (RID), enabling interactions with Rab GTPases, particularly Rab8 and Rab11. which regulate intracellular vesicle trafficking and organelle positioning. RILPL1 plays critical roles in ciliogenesis, cell division, and secretory pathways by modulating centrosome positioning, ciliary membrane formation, and microtubule dynamics.
Notably, RILPL1 is implicated in LRRK2 (leucine-rich repeat kinase 2)-linked Parkinson’s disease pathology. Mutations in LRRK2 hyperphosphorylate RILPL1. disrupting its interaction with Rab GTPases and impairing cilia formation, potentially contributing to neurodegenerative mechanisms. Dysregulated RILPL1 expression is also associated with cancers and ciliopathies, underscoring its importance in cellular homeostasis.
Structurally, RILPL1 shares homology with RILP but lacks the lysosomal-targeting domain, localizing instead to centrosomes or ciliary bases. Its recombinant form enables studies on Rab-mediated trafficking, cilia-related disorders, and therapeutic targeting. Current research focuses on elucidating its precise molecular interactions and role in disease pathways, positioning RILPL1 as a potential biomarker or intervention target.
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