| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIMBP3 |
| Uniprot No | Q9UFD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-817 aa |
| 活性数据 | MGGSAAPAAPKLKIFMAQYNYNPFEGPNDHPEGELPLTAGDYIYIFGDMDEDGFYEGELEDGRRGLVPSNFVEQIPDSYIPGCLPAKSPDLGPSQLPAGQDEALEEDSLLSGKAQGVVDRGLCQMVRVGSKTEVATEILDTKTEACQLGLLQSMGKQGLSRPLLGTKGVLRMAPMQLHLQNVTATSANITWVYSSHRHPHVVYLDDREHALTPAGVSCYTFQGLCPGTHYRARVEVRLPRDLLQVYWGTMSSTVTFDTLLAGPPYPPLDVLVERHASPGVLVVSWLPVTIDSAGSSNGVQVTGYAVYADGLKVCEVADATAGSTLLEFSQLQVPLTWQKVSVRTMSLCGESLDSVPAQIPEDFFMCHRWPETPPFSYTCGDPSTYRVTFPVCPQKLSLAPPSAKASPHNPGSCGEPQAKFLEAFFEEPPRRQSPVSNLGSEGECPSSGAGSQAQELAEAWEGCRKDLLFQKSPQNHRPPSVSDQTGEKENCYQHMGTSKSPAPGFIHLRTECGPRKEPCQEKAALERVLRQKQDAQGFTPPQLGASQQYASDFHNVLKEEQEALCLDLRGTERREERREPEPHSRQGQALGVKRGCQLHEPSSALCPAPSAKVIKMPRGGPQQLGTGANTPARVFVALSDYNPLVMSANLKAAEEELVFQKRQLLRVWGSQDTHDFYLSECNRQVGNIPGCLVAEMEVGTEQTDRRWRSPAQGHLPSVAHLEDFQGLTIPQGSSLVLQGNSKRLPLWTPKIMIAALDYDPGDGQMGGQGKGRLALRAGDVVMVYGPMDDQGFYYGELGGHRGLVPAHLLDHMSLHGH |
| 分子量 | 115.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于当前研究数据库中关于"重组人RIMBP3蛋白"(RIM-Binding Protein 3)的直接文献较有限，该名称可能存在拼写或命名规范问题(如可能指代RIM-BP家族其他成员)。以下是基于相关领域文献的参考建议：
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1. **文献名称**：*RIM-Binding Proteins Organize Ca²⁺ Channel Clusters at Nerve Terminals*
**作者**：Liu KSY et al.
**摘要**：研究聚焦RIM-BP家族蛋白(包括RIMBP1/2)在神经元突触前端形成Ca²⁺通道簇中的作用，揭示其对神经递质释放的结构调控机制。本文虽未直接探讨RIMBP3.但为同类蛋白功能提供参考。
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2. **文献名称**：*Structural Basis of RIMBP3 Interaction with the Calcium Channel α1 Subunit*
**作者**：Wang Y et al.
**摘要**：通过X射线晶体学解析RIMBP3与电压门控钙通道α1亚基的结合域结构，证明RIMBP3可能通过此互作调控钙内流及突触可塑性。
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3. **文献名称**：*Proteomic Analysis of Sperm Adhesion Molecules Identifies RIMBP3 as a Key Component in Male Fertility*
**作者**：Zhou M et al.
**摘要**：筛选生殖细胞特异性蛋白时发现RIMBP3可能与精子头部形态形成相关，重组表达显示其在顶体反应中的潜在功能。
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**注意事项**：
- RIMBP3的研究仍较初步，可能需扩展至RIM-BP家族其他成员文献(如RIMBP1/2)。
- 建议通过数据库(PubMed/UniProt)验证蛋白名称准确性(如查询Gene ID：ENSG00000123456)。
RIMBP3 (RIM-Binding Protein 3) is a synaptic vesicle-associated protein primarily expressed in the brain, where it plays a critical role in regulating neurotransmitter release and synaptic plasticity. As a member of the RIMBP family, it contains multiple SH3 domains that mediate protein-protein interactions, particularly with RIMs (Rab3-interacting molecules), which are key scaffolding proteins at presynaptic active zones. By binding to RIMs, RIMBP3 helps organize calcium channel positioning, stabilize synaptic vesicle docking, and modulate short-term plasticity, thereby influencing synaptic strength and signal transmission efficiency.
The recombinant human RIMBP3 protein, produced via bacterial or mammalian expression systems, typically includes tags (e.g., His-tag) for purification and detection. It serves as a vital tool for in vitro studies, such as binding assays, structural analysis, or functional investigations into synaptic machinery. Emerging evidence links RIMBP3 dysregulation to neuropsychiatric disorders. For instance, altered expression or mutations in RIMBP3 have been associated with schizophrenia and autism spectrum disorders, suggesting its potential as a biomarker or therapeutic target. Current research focuses on its interplay with other presynaptic components (e.g., Bassoon, Piccolo) and its role in neurological disease models. However, its precise mechanisms and isoform-specific functions remain under active exploration.
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