| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIMBP2 |
| Uniprot No | O15034 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-645 aa |
| 活性数据 | MNGLATSLGKGQESAIGGSSAIGEYIRPLPQPGDRPEPLSAKPTFLSRSGSARCRSESDMENERNSNTSKQRYSGKVHLCVARYSYNPFDGPNENPEAELPLTAGKYLYVYGDMDEDGFYEGELLDGQRGLVPSNFVDFVQDNESRLASTLGNEQDQNFINHSGIGLEGEHILDLHSPTHIDAGITDNSAGTLDVNIDDIGEDIVPYPRKITLIKQLAKSVIVGWEPPAVPPGWGTVSSYNVLVDKETRMNLTLGSRTKALIEKLNMAACTYRISVQCVTSRGSSDELQCTLLVGKDVVVAPSHLRVDNITQISAQLSWLPTNSNYSHVIFLNEEEFDIVKAARYKYQFFNLRPNMAYKVKVLAKPHQMPWQLPLEQREKKEAFVEFSTLPAGPPAPPQDVTVQAGVTPATIRVSWRPPVLTPTGLSNGANVTGYGVYAKGQRVAEVIFPTADSTAVELVRLRSLEAKGVTVRTLSAQGESVDSAVAAVPPELLVPPTPHPRPAPQSKPLASSGVPETKDEHLGPHARMDEAWEQSRAPGPVHGHMLEPPVGPGRRSPSPSRILPQPQGTPVSTTVAKAMAREAAQRVAESSRLEKRSVFLERSSAGQYAASDEEDAYDSPDFKRRGASVDDFLKGSELGKQMVS |
| 分子量 | 71 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RIMBP2蛋白的3篇文献概览(经格式简化，内容整合自相关研究领域)：
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1. **文献名称**：*RIMBP2 maintains active zone stability by anchoring synaptic vesicles to the cytoskeleton*
**作者**：Smith A, et al.
**摘要**：研究发现RIMBP2通过与RIM蛋白及细胞骨架蛋白相互作用，维持突触前活跃区的结构稳定性。利用重组RIMBP2蛋白的体外实验表明，其C末端结构域直接结合微管蛋白，调控突触囊泡的锚定与神经递质释放效率。
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2. **文献名称**：*Structural analysis of human RIMBP2 reveals a conserved role in sperm acrosome formation*
**作者**：Zhang Y, et al.
**摘要**：通过重组表达人RIMBP2的多个截短体，解析了其N端卷曲螺旋结构域与精子顶体膜蛋白互作的分子机制。实验表明RIMBP2缺失会导致顶体形态异常，提示其在生殖细胞发育中的关键作用。
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3. **文献名称**：*Proteomic screening identifies RIMBP2 as a novel glioblastoma biomarker*
**作者**：Chen L, et al.
**摘要**：基于重组RIMBP2蛋白制备的单克隆抗体，在胶质母细胞瘤组织中发现RIMBP2异常高表达。功能实验显示RIMBP2通过激活MAPK通路促进肿瘤侵袭，可能成为治疗靶点。
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**备注**：以上内容为模拟概括，实际文献需通过PubMed/Google Scholar检索关键词(如"RIMBP2 recombinant"、"RIMBP2 function")获取。RIMBP2研究多集中于神经突触、生殖细胞及部分癌症领域，其重组蛋白常用于结构互作或疾病机制研究。
**Background of Recombinant Human RIMBP2 Protein**
Recombinant human RIM-binding protein 2 (RIMBP2) is a multidomain scaffolding protein involved in regulating synaptic vesicle release and cellular adhesion. A member of the RIM-BP family, it interacts with Rab3-interacting molecules (RIMs) and voltage-gated calcium channels, playing a pivotal role in organizing the presynaptic active zone—a specialized site for neurotransmitter release. Structurally, RIMBP2 contains three SH3 domains and two fibronectin type III (FNIII) domains, enabling multivalent interactions with synaptic proteins to maintain synaptic architecture and modulate exocytosis.
RIMBP2 is highly expressed in the brain and testis, suggesting dual roles in neuronal signaling and germ cell development. Dysregulation of RIMBP2 has been implicated in neurological disorders and cancers, particularly those linked to synaptic dysfunction or chromosomal instability. Recombinant RIMBP2. produced via heterologous expression systems (e.g., bacteria, mammalian cells), facilitates in vitro studies on its binding partners, structural dynamics, and functional mechanisms. Its recombinant form is essential for dissecting molecular pathways in synaptic plasticity, spermatogenesis, and disease pathology, offering potential therapeutic insights.
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