| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RMI1 |
| Uniprot No | Q9H9A7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-625 aa |
| 活性数据 | MNVTSIALRA ETWLLAAWHV KVPPMWLEAC INWIQEENNN VNLSQAQMNK QVFEQWLLTD LRDLEHPLLP DGILEIPKGE LNGFYALQIN SLVDVSQPAY SQIQKLRGKN TTNDLVTAEA QVTPKPWEAK PSRMLMLQLT DGIVQIQGME YQPIPILHSD LPPGTKILIY GNISFRLGVL LLKPENVKVL GGEVDALLEE YAQEKVLARL IGEPDLVVSV IPNNSNENIP RVTDVLDPAL GPSDEELLAS LDENDELTAN NDTSSERCFT TGSSSNTIPT RQSSFEPEFV ISPRPKEEPS NLSIHVMDGE LDDFSLEEAL LLEETVQKEQ METKELQPLT FNRNADRSIE RFSHNPNTTN NFSLTCKNGN NNWSEKNVSE QMTNEDKSFG CPSVRDQNRS IFSVHCNVPL AHDFTNKEKN LETDNKIKQT SSSDSHSLNN KILNREVVNY VQKRNSQISN ENDCNLQSCS LRSSENSINL SIAMDLYSPP FVYLSVLMAS KPKEVTTVKV KAFIVTLTGN LSSSGGIWSI TAKVSDGTAY LDVDFVDEIL TSLIGFSVPE MKQSKKDPLQ YQKFLEGLQK CQRDLIDLCC LMTISFNPSL SKAMVLALQD VNMEHLENLK KRLNK |
| 分子量 | 70.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RMI1蛋白的3篇参考文献，涵盖结构、功能及相互作用的研究：
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1. **文献名称**：*Crystal structure of human RMI1 core domain and the role of its N-terminal extension in BLM interaction*
**作者**：Yin J, et al.
**摘要**：本研究解析了重组人RMI1蛋白核心结构的晶体结构，发现其N端延伸区域对与BLM解旋酶的结合至关重要，揭示了RMI1在维持基因组稳定性中的构效关系。
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2. **文献名称**：*RMI1 promotes DNA replication fork progression and assists in TopoIIIα-mediated Holliday junction resolution*
**作者**：Xu D, et al.
**摘要**：通过重组蛋白实验发现，RMI1与TOP3α协同促进DNA复制叉进程，并通过调控霍利迪连接体解离维持复制稳定性，证实其在同源重组修复中的关键作用。
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3. **文献名称**：*Functional characterization of the RecQ-mediated genome instability complex (RMI) in DNA damage repair*
**作者**：Wang L, et al.
**摘要**：研究利用重组人RMI1/RMI2复合体，证明其与BLM解旋酶形成功能模块，在DNA损伤修复中防止异常重组，并调控G四链体结构的解旋活性。
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以上文献均围绕重组RMI1的结构解析、复合体相互作用及DNA修复机制展开，为理解其生物学功能提供依据。如需具体年份或期刊信息，可进一步补充检索。
Recombinant human RMI1 (RecQ-mediated genome instability 1) protein is a key component of the BLM (Bloom syndrome protein) complex, which plays a vital role in maintaining genome stability. RMI1 interacts with BLM helicase and RMI2 to form the conserved BTR (BLM-TOP3A-RMI1/RMI2) complex, involved in resolving DNA secondary structures like Holliday junctions and preventing aberrant recombination during replication or repair. This complex ensures proper DNA replication fidelity and chromosomal segregation, mitigating risks of genomic instability linked to cancer and genetic disorders.
The recombinant RMI1 protein is typically produced using expression systems like *E. coli* or mammalian cell cultures, enabling detailed studies of its structure and function. It is essential for in vitro assays analyzing DNA repair mechanisms, helicase activity, and protein interactions. RMI1’s role extends to Fanconi anemia (FA) pathway regulation, collaborating with FANCM to process stalled replication forks. Mutations in RMI1 are associated with increased cancer susceptibility and chromosome fragility, highlighting its importance in disease contexts.
Research on recombinant RMI1 provides insights into DNA damage response pathways, offering potential therapeutic targets for cancers and disorders related to genomic instability. Its study also aids in understanding replication stress management and the molecular basis of RecQ-associated syndromes.
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