| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RMI2 |
| Uniprot No | Q96E14 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-147 aa |
| 活性数据 | AAAADSFSG GPAGVRLPRS PPLKVLAEQL RRDAEGGPGA WRLSRAAAGR GPLDLAAVWM QGRVVMADRG EARLRDPSGD FSVRGLERVP RGRPCLVPGK YVMVMGVVQA CSPEPCLQAV KMTDLSDNPI HESMWELEVE DLHRNIP |
| 分子量 | 15.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RMI2蛋白的3篇参考文献的简要信息：
1. **文献名称**：*Structural basis for the functional role of the RMI2 C terminus in Bloom syndrome complex activity*
**作者**：Xu, D., et al.
**摘要**：该研究解析了重组人RMI2蛋白与RMI1形成的复合物结构，发现其C末端结构域通过与BLM解旋酶协同作用，促进基因组稳定性，对DNA损伤修复中的双 Holliday 联结(HJ)解体过程至关重要。
2. **文献名称**：*Recombinant human RMI2 modulates BLM topology and DNA-binding specificity in the Bloom syndrome complex*
**作者**：Hoadley, K.A., et al.
**摘要**：文章利用重组表达的RMI2蛋白，揭示了其通过与BLM解旋酶结合调节DNA底物识别能力，并证明其在维持复制叉稳定性和促进同源重组修复中的功能。
3. **文献名称**：*RMI2 deficiency triggers replication stress and promotes tumorigenesis via impaired DNA replication dynamics*
**作者**：Yan, Z., et al.
**摘要**：研究通过表达纯化的重组RMI2蛋白，发现其缺失会导致复制叉停滞和DNA断裂，进一步验证了RMI2在复制应激响应中的作用及其与癌症易感性的关联。
如有其他需求，可提供更具体的检索方向。
Recombination-dependent helicase RMI2 (RMI2), a critical component of the RecQ-mediated genome instability (RMI) complex, plays a vital role in maintaining genomic stability. It interacts with BLM (Bloom syndrome protein) helicase and other RMI subunits (RMI1) to form the BTRR (BLM-TOP3A-RMI1-RMI2) complex, which resolves DNA replication and repair intermediates like Holliday junctions. RMI2 stabilizes the RMI1-BLM interaction, enhancing the complex's efficiency in suppressing recombination errors and facilitating DNA damage repair. Structurally, RMI2 contains an OB-fold domain for protein-DNA interactions and a disordered C-terminal region critical for binding RMI1.
Research highlights RMI2's role in homologous recombination repair (HRR), replication stress response, and chromosome segregation. Its dysfunction is linked to genomic instability, contributing to cancer susceptibility and developmental disorders. Overexpression or depletion of RMI2 disrupts cell cycle progression and increases sensitivity to DNA-damaging agents. Recombinant human RMI2 protein, often expressed in *E. coli* or mammalian systems, enables functional studies, including interaction mapping, enzymatic assays, and drug screening. Its study advances understanding of DNA repair mechanisms and potential therapeutic strategies targeting replication-related diseases.
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