| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF170 |
| Uniprot No | Q96K19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200 aa |
| 活性数据 | MAKYQGEVQSLKLDDDSVIEGVSDQVLVAVVVSFALIATLVYALFRNVHQNIHPENQELVRVLREQLQTEQDAPAATRQQFYTDMYCPICLHQASFPVETNCGHLFCGACIIAYWRYGSWLGAISCPICRQTGSSEKSSRASEQTHQEAVACLDTQNSPACTVGCRSGPQHIPHDRMLPSASPRLCFTLLDCVSILWFSG |
| 分子量 | 48.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNF170蛋白的3篇代表性文献的简要总结(基于公开研究信息模拟整理，非真实文献)：
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1. **文献名称**: *RNF170 modulates ubiquitination and endoplasmic reticulum-associated degradation in neuronal cells*
**作者**: Smith A, et al. (2015)
**摘要**: 该研究揭示了RNF170作为E3泛素连接酶，通过调控内质网相关蛋白的泛素化修饰参与蛋白质质量控制，其功能异常可能引发神经退行性疾病相关通路紊乱。
2. **文献名称**: *RNF170 regulates calcium signaling by promoting the degradation of type 1 inositol 1.4.5-trisphosphate receptors*
**作者**: Johnson B, et al. (2018)
**摘要**: 本文发现RNF170通过泛素化降解IP3R1受体蛋白，影响细胞内钙离子稳态，揭示了其在阿尔茨海默病等钙信号失调疾病中的潜在作用机制。
3. **文献名称**: *Structural analysis of human RNF170 reveals a conserved ring domain essential for ubiquitin ligase activity*
**作者**: Chen L, et al. (2020)
**摘要**: 通过X射线晶体学解析了RNF170蛋白的环状结构域(RING domain)的三维结构，并验证了其与泛素结合酶协同催化底物泛素化的分子基础。
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**注**：以上为模拟简化文献示例，实际研究需以真实数据库(如PubMed)的检索结果为准。建议通过关键词“RNF170”“ubiquitination”或“neurodegeneration”在学术平台查找具体文献。
Recombinant human RNF170 (Ring Finger Protein 170) is a ubiquitously expressed E3 ubiquitin ligase belonging to the RING finger protein family. It contains a characteristic RING domain at its N-terminus, which facilitates interactions with E2 ubiquitin-conjugating enzymes to mediate substrate-specific protein ubiquitination—a key process in proteasomal degradation, DNA repair, and cellular signaling. Structurally, RNF170 also features a transmembrane domain, anchoring it to the endoplasmic reticulum (ER) membrane, where it participates in ER-associated degradation (ERAD) pathways by targeting misfolded proteins for disposal.
Research highlights RNF170's role in neurological health, particularly in hereditary spastic paraplegia (HSP), where mutations in the RNF170 gene (linked to HSP type SPG18) disrupt ER homeostasis and calcium signaling, leading to progressive motor neuron degeneration. Studies suggest it may regulate inositol 1.4.5-trisphosphate receptors (IP3Rs), influencing calcium release from ER stores. Recombinant RNF170 is utilized to study these molecular mechanisms, offering insights into neuropathology and potential therapeutic targets. Its biochemical properties and interactions (e.g., with VCP/p97 in ERAD) remain active areas of investigation to clarify its dual roles in protein quality control and disease pathogenesis.
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