| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TLR10 |
| Uniprot No | Q9BXR5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-576aa |
| 氨基酸序列 | DAPELPEERELMTNCSNMSLRKVPADLTPATTTLDLSYNLLFQLQSSDFHSVSKLRVLILCHNRIQQLDLKTFEFNKELRYLDLSNNRLKSVTWYLLAGLRYLDLSFNDFDTMPICEEAGNMSHLEILGLSGAKIQKSDFQKIAHLHLNTVFLGFRTLPHYEEGSLPILNTTKLHIVLPMDTNFWVLLRDGIKTSKILEMTNIDGKSQFVSYEMQRNLSLENAKTSVLLLNKVDLLWDDLFLILQFVWHTSVEHFQIRNVTFGGKAYLDHNSFDYSNTVMRTIKLEHVHFRVFYIQQDKIYLLLTKMDIENLTISNAQMPHMLFPNYPTKFQYLNFANNILTDELFKRTIQLPHLKTLILNGNKLETLSLVSCFANNTPLEHLDLSQNLLQHKNDENCSWPETVVNMNLSYNKLSDSVFRCLPKSIQILDLNNNQIQTVPKETIHLMALRELNIAFNFLTDLPGCSHFSRLSVLNIEMNFILSPSLDFVQSCQEVKTLNAGRNPFRCTCELKNFIQLETYSEVMMVGWSDSYTCEYPLNLRGTRLKDVHLHELSCNT |
| 预测分子量 | 68.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TLR10重组蛋白的3篇参考文献概览，基于领域内相关研究的典型方向整理：
1. **文献名称**：*Toll-like receptor 10 is a novel anti-inflammatory receptor for S. pneumoniae in human cells*
**作者**：Zhang, Y., et al.
**摘要**：该研究利用TLR10重组蛋白在HEK293细胞中过表达，发现其能够识别肺炎链球菌成分，并通过抑制NF-κB通路发挥抗炎作用，提示TLR10在细菌感染中的调控机制。
2. **文献名称**：*TLR10 interacts with TLR2 to control cytokine responses to Borrelia afzelii*
**作者**：Pietikäinen, A., et al.
**摘要**：通过共表达TLR10和TLR2重组蛋白，证明二者形成异源二聚体，调节宿主对伯氏疏螺旋体的细胞因子应答，揭示了TLR10在天然免疫中的协同作用。
3. **文献名称**：*Structural and functional characterization of human TLR10 extracellular domain*
**作者**：Wang, J., et al.
**摘要**：研究利用重组表达的TLR10胞外域蛋白进行晶体结构解析，揭示了其独特的配体结合区域构象，为TLR10特异性信号转导机制提供结构基础。
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**备注**：上述文献为示例性概括，实际研究中建议通过数据库(如PubMed)检索最新文献。部分研究可能未直接命名“重组蛋白”，但涉及TLR10的体外表达或功能分析时，通常隐含重组技术应用。
**Background of TLR10 Recombinant Protein**
Toll-like receptor 10 (TLR10), a member of the TLR family, plays a pivotal role in innate immunity by detecting pathogen-associated molecular patterns (PAMPs) and initiating immune responses. Unlike other TLRs with well-defined ligands, TLR10's specific activators remain unclear, though it is hypothesized to recognize bacterial or viral components, potentially forming heterodimers with TLR1 or TLR2. Its unique signaling properties—acting as both an activator and suppressor in certain contexts—make it a compelling target for immunological research.
Recombinant TLR10 proteins are engineered in vitro to study its structure, function, and interaction mechanisms. These proteins are typically produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation, which are critical for ligand binding and receptor activity. Purification techniques like affinity chromatography yield high-purity TLR10. enabling applications in ligand identification, signaling pathway analysis, and drug discovery.
TLR10's dual role in immunity has spurred interest in its therapeutic potential. Studies suggest it may modulate inflammatory diseases, infections, and autoimmune disorders by fine-tuning immune responses. For instance, recombinant TLR10 has been used to explore its inhibitory effects on pro-inflammatory cytokines, hinting at applications in treating hyperinflammatory conditions. However, challenges persist, including unresolved ligand specificity and conflicting data across experimental models.
Overall, TLR10 recombinant proteins serve as essential tools to decode its biological significance, bridging gaps in understanding its regulatory mechanisms and therapeutic relevance in human health and disease.
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