| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF186 |
| Uniprot No | Q9NXI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227 aa |
| 活性数据 | MACTKTLQQSQPISAGATTTTTAVAPAGGHSGSTECDLECLVCREPYSCPRLPKLLACQHAFCAICLKLLLCVQDNTWSITCPLCRKVTAVPGGLICSLRDHEAVVGQLAQPCTEVSLCPQGLVDPADLAAGHPSLVGEDGQDEVSANHVAARRLAAHLLLLALLIILIGPFIYPGVLRWVLTFIIALALLMSTLFCCLPSTRGSCWPSSRTLFCREQKHSHISSIA |
| 分子量 | 50.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人RNF186蛋白的3篇参考文献概览(虚构示例，仅作格式参考)：
1. **文献名称**：*RNF186 regulates mitochondrial autophagy and inflammatory response in ulcerative colitis*
**作者**：Smith A, et al.
**摘要**：研究揭示RNF186通过调控线粒体自噬途径抑制肠道炎症，敲除小鼠模型显示其缺失加剧结肠炎，并促进NLRP3炎症小体激活。
2. **文献名称**：*Structural and functional analysis of RNF186 E3 ubiquitin ligase domain*
**作者**：Chen L, et al.
**摘要**：解析了RNF186蛋白的RING结构域晶体结构，证实其具有泛素化活性，并发现其通过泛素化修饰调节肠道上皮细胞屏障功能。
3. **文献名称**：*RNF186 interacts with ATG16L1 to modulate Crohn’s disease susceptibility*
**作者**：Tanaka K, et al.
**摘要**：发现RNF186与自噬蛋白ATG16L1直接互作，影响胞内病原体清除能力，其基因多态性与克罗恩病风险显著相关。
*注：以上文献为模拟数据，实际研究中建议通过PubMed或Google Scholar以“RNF186”为关键词检索具体论文。*
RNF186 (Ring Finger Protein 186) is a human protein encoded by the RNF186 gene, belonging to the RING-type E3 ubiquitin ligase family. It contains a conserved RING domain at its N-terminus, facilitating interactions with E2 ubiquitin-conjugating enzymes to mediate substrate ubiquitination—a key post-translational modification regulating protein degradation, trafficking, or signaling. Predominantly expressed in the gastrointestinal tract, RNF186 localizes to membrane-bound compartments, including the endoplasmic reticulum and plasma membrane, suggesting roles in membrane-associated processes.
Functionally, RNF186 is implicated in maintaining intestinal homeostasis and mucosal immunity. Genome-wide association studies (GWAS) link RNF186 polymorphisms to inflammatory bowel diseases (IBD), particularly Crohn’s disease and ulcerative colitis. It modulates autophagy, a critical mechanism for clearing intracellular pathogens and mitigating endoplasmic reticulum stress, by ubiquitinating autophagy-related proteins. Additionally, RNF186 interacts with components of the inflammasome and immune signaling pathways, influencing cytokine production and epithelial barrier integrity.
Recombinant RNF186 protein is utilized to study its biochemical properties, substrate specificity, and interactions in vitro. Its therapeutic potential is being explored for IBD treatment, leveraging its regulatory effects on inflammation and epithelial repair. However, mechanistic details remain under investigation, particularly its tissue-specific roles and interplay with gut microbiota. Understanding RNF186’s function could unlock novel strategies for managing autoimmune and inflammatory disorders.
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