| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF184 |
| Uniprot No | Q9HCI7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-577 aa |
| 活性数据 | MNPVNATALYISASRLVLNYDPGDPKAFTEINRLLPYFRQSLSCCVCGHLLQDPIAPTNSTCQHYVCKTCKGKKMMMKPSCSWCKDYEQFEENKQLSILVNCYKKLCEYITQTTLARDIIEAVDCSSDILALLNDGSLFCEETEKPSDSSFTLCLTHSPLPSTSEPTTDPQASLSPMSESTLSIAIGSSVINGLPTYNGLSIDRFGINIPSPEHSNTIDVCNTVDIKTEDLSDSLPPVCDTVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETVSNTEVCCPNLQPNLEATVSNGPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKIAKLNRKRSRSESDSEKVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSHSKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKACLDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAASTHDDKSLDEAIDMRFDC |
| 分子量 | 88.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNF184蛋白的3篇参考文献示例(文献信息为模拟概括，仅供参考)：
1. **文献名称**: *RNF184 mediates proteasomal degradation of synaptic proteins and is linked to neurodegenerative disorders*
**作者**: Chen L, et al.
**摘要**: 该研究阐明了重组人RNF184作为E3泛素连接酶的功能，证明其通过泛素化修饰突触相关蛋白(如PSD-95)，促进其蛋白酶体降解。实验表明，RNF184在阿尔茨海默病患者脑组织中表达上调，可能通过破坏突触稳定性加速神经退行性病变。
2. **文献名称**: *Recombinant human RNF184 suppresses prostate cancer progression via ubiquitination-dependent EGFR signaling inhibition*
**作者**: Wang Y, et al.
**摘要**: 研究利用重组表达的RNF184蛋白，发现其通过泛素化修饰EGFR并促进其溶酶体降解，从而抑制前列腺癌细胞增殖和迁移。体内实验证实，RNF184过表达显著延缓肿瘤生长，提示其作为癌症治疗靶点的潜力。
3. **文献名称**: *Structural and functional analysis of the RING domain in recombinant human RNF184*
**作者**: Zhang Q, et al.
**摘要**: 该研究解析了重组人RNF184蛋白的RING结构域晶体结构，鉴定出关键锌离子结合位点。功能实验表明，该结构域突变会破坏其E3泛素连接酶活性，并影响其与底物蛋白(如HIF-1α)的相互作用，揭示了RNF184在缺氧应答中的调控机制。
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**注**：以上内容为基于领域知识的合理推测，实际文献需通过数据库(如PubMed/Web of Science)以“RNF184”或“recombinant RNF184”为关键词检索获取。若需具体文献，建议结合最新研究进行定向查阅。
RNF184 (Ring Finger Protein 184) is a member of the RING finger protein family, characterized by a conserved cysteine-rich zinc-binding domain involved in protein-protein interactions and ubiquitination processes. Encoded by the RNF184 gene located on human chromosome 7p22.1. this E3 ubiquitin ligase plays a regulatory role in the ubiquitin-proteasome system, mediating substrate-specific protein degradation. Structurally, RNF184 contains a C-terminal RING domain critical for its enzymatic activity and an N-terminal region that may facilitate substrate recognition.
Expression profiling indicates RNF184 is predominantly expressed in the brain, particularly in regions like the cerebellum and hippocampus, suggesting a potential role in neuronal function. Studies also report its presence in testis and some cancer tissues, though functional implications remain unclear. Emerging evidence links RNF184 to neurodegenerative diseases, notably Alzheimer’s disease, where it may influence tau protein stability and autophagic clearance pathways. Additionally, it interacts with mitochondrial proteins, hinting at a role in mitochondrial quality control and oxidative stress responses.
Despite its biological significance, RNF184 remains understudied compared to other RING family members. Current research focuses on elucidating its substrates, signaling networks, and disease-related mechanisms. Its dual association with ubiquitination and cellular stress pathways positions RNF184 as a potential therapeutic target for neurodegeneration and metabolic disorders, warranting further exploration.
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