| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF180 |
| Uniprot No | Q86T96 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-416 aa |
| 活性数据 | MKRSKELITKNHSQEETSILRCWKCRKCIASSGCFMEYLENQVIKDKDDSVDAQNICHVWHMNVEALPEWISCLIQKAQWTVGKLNCPFCGARLGGFNFVSTPKCSCGQLAAVHLSKSRTDYQPTQAGRLMRPSVKYLSHPRVQSGCDKEALLTGGGSENRNHRLLNMARNNNDPGRLTEALCLEVRPTYFEMKNEKLLSKASEPKYQLFVPQLVTGRCATRAFHRKSHSLDLNISEKLTLLPTLYEIHSKTTAYSRLNETQPIDLSGLPLQSSKNSYSFQNPSSFDPSMLLQRFSVAPHETQTQRGGEFQCGLEAASVYSDHTNTNNLTFLMDLPSAGRSMPEASDQEEHLSPLDFLHSANFSLGSINQRLNKRERSKLKNLRRKQRRRERWLQKQGKYSGVGLLDHMVSIYLLI |
| 分子量 | 73.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的3篇关于重组人RNF180蛋白的研究文献概要(均为模拟示例，实际文献需根据具体数据库检索补充)：
---
1. **文献名称**: 《RNF180甲基化作为胃癌诊断的新型血清标志物》
**作者**: Zhang L. et al. (2018)
**摘要**: 研究揭示RNF180基因启动子区异常甲基化与胃癌发生相关，其蛋白表达下调可能作为胃癌早期诊断的潜在血清标志物，并通过泛素化修饰调控肿瘤细胞凋亡通路。
2. **文献名称**: 《RNF180通过泛素化Wnt通路抑制结直肠癌转移》
**作者**: Wang Y. et al. (2020)
**摘要**: 实验表明RNF180蛋白在结直肠癌中显著低表达，过表达RNF180可通过泛素化降解β-catenin蛋白，抑制Wnt/β-catenin信号通路活性，从而减缓肿瘤细胞增殖和迁移。
3. **文献名称**: 《RNF180在阿尔茨海默病中的表观遗传调控作用》
**作者**: Chen R. et al. (2019)
**摘要**: 首次报道RNF180在阿尔茨海默病患者脑组织中的异常低表达，其启动子高甲基化状态可能导致神经突触相关蛋白泛素化失调，与Tau蛋白异常聚集相关。
---
如需具体文献，建议通过PubMed、Web of Science等平台以“RNF180 protein”、“ubiquitination”、“cancer”或“neurodegeneration”为关键词检索，重点关注其泛素连接酶功能及在疾病中的调控机制。
Recombinant human RNF180 (Ring Finger Protein 180) is a ubiquitin-protein ligase encoded by the RNF180 gene, located on chromosome 5q12.3. As a member of the RING finger protein family, it contains a characteristic RING-H2 domain that facilitates E3 ubiquitin ligase activity, mediating substrate ubiquitination and subsequent degradation via the proteasome. RNF180 is predominantly expressed in the brain, particularly in neurons, suggesting roles in neural development, synaptic function, or neurodegenerative processes. Studies have linked it to neuropsychiatric disorders, including schizophrenia and Alzheimer’s disease, though its precise mechanisms remain under investigation.
Recombinant RNF180 is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to produce purified protein for functional and structural studies. This enables research into its interaction partners, enzymatic regulation, and post-translational modifications. Its role in apoptosis, cell cycle regulation, and tumor suppression has also been explored, with evidence showing altered expression in certain cancers, hinting at potential therapeutic applications. Structural analysis reveals conserved domains critical for substrate recognition and ubiquitin transfer, making recombinant RNF180 valuable for elucidating disease pathways and drug discovery. Ongoing research aims to clarify its physiological substrates and pathological implications across tissues.
×
