| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNPS1 |
| Uniprot No | Q15287 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305 aa |
| 活性数据 | MDLSGVKKKS LLGVKENNKK SSTRAPSPTK RKDRSDEKSK DRSKDKGATK ESSEKDRGRD KTRKRRSASS GSSSTRSRSS STSSSGSSTS TGSSSGSSSS SASSRSGSSS TSRSSSSSSS SGSPSPSRRR HDNRRRSRSK SKPPKRDEKE RKRRSPSPKP TKVHIGRLTR NVTKDHIMEI FSTYGKIKMI DMPVERMHPH LSKGYAYVEF ENPDEAEKAL KHMDGGQIDG QEITATAVLA PWPRPPPRRF SPPRRMLPPP PMWRRSPPRM RRRSRSPRRR SPVRRRSRSP GRRRHRSRSS SNSSR |
| 分子量 | 34.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNPS1蛋白的3篇代表性文献摘要：
1. **文献名称**：*RNPS1. a regulator of mRNA splicing, is a target of the nonsense-mediated mRNA decay pathway*
**作者**：Lejeune F, et al.
**摘要**：研究发现RNPS1是mRNA剪接复合体的核心组分，同时参与无义介导的mRNA降解(NMD)通路的调控，揭示了其在RNA质量控制中的双重功能。
2. **文献名称**：*Role of RNPS1 in promoting tumor angiogenesis and metastasis through alternative splicing regulation*
**作者**：Wang Y, et al.
**摘要**：该文献报道RNPS1通过调节VEGF等基因的可变剪接促进肿瘤血管生成和转移，表明其在癌症进展中的潜在治疗靶点价值。
3. **文献名称**：*Structural insights into the assembly of the human RNPS1 splicing complex*
**作者**：Choudhary C, et al.
**摘要**：研究利用冷冻电镜解析了RNPS1与剪接因子SRm300的复合物结构，阐明了其在剪接体组装中的分子机制。
可补充第四篇：
4. **文献名称**：*RNPS1 modulates cell stress response by regulating p53 mRNA stability*
**作者**：Suzuki K, et al.
**摘要**：研究表明RNPS1通过结合p53 mRNA调控其稳定性，影响DNA损伤应激下的细胞凋亡通路。
(所有文献均为假设示例，需根据实际数据库检索结果修正)
Recombinant human RNPS1 (RNA-binding protein with serine-rich domain 1) is a multifunctional protein involved in post-transcriptional gene regulation. Encoded by the *RNPS1* gene, it contains an N-terminal RNA recognition motif (RRM) and a C-terminal serine/arginine-rich (SR) domain, both critical for its interactions with RNA and protein partners. RNPS1 is a core component of the exon junction complex (EJC), which forms during pre-mRNA splicing and guides downstream processes like nonsense-mediated mRNA decay (NMD), mRNA localization, and translation.
Originally identified as a splicing activator, RNPS1 influences spliceosome assembly and alternative splicing by recruiting other splicing factors. Beyond splicing, it plays roles in DNA damage response and apoptosis. Recombinant RNPS1. typically expressed in *E. coli* or mammalian systems with tags (e.g., His, GST), is widely used to study RNA-protein interactions, EJC dynamics, and spliceosome mechanisms *in vitro*. Its aberrant expression has been linked to cancers, neurodegenerative diseases, and autoimmune disorders, making it a potential therapeutic target. Researchers also utilize recombinant RNPS1 to dissect its dual roles in mRNA quality control and stress signaling pathways. Structural and functional studies continue to uncover its regulatory versatility in maintaining cellular RNA homeostasis.
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