| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF6 |
| Uniprot No | Q9Y252 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-685 aa |
| 活性数据 | MNQSRSRSDG GSEETLPQDH NHHENERRWQ QERLHREEAY YQFINELNDE DYRLMRDHNL LGTPGEITSE ELQQRLDGVK EQLASQPDLR DGTNYRDSEV PRESSHEDSL LEWLNTFRRT GNATRSGQNG NQTWRAVSRT NPNNGEFRFS LEIHVNHENR GFEIHGEDYT DIPLSDSNRD HTANRQQRST SPVARRTRSQ TSVNFNGSSS NIPRTRLASR GQNPAEGSFS TLGRLRNGIG GAAGIPRANA SRTNFSSHTN QSGGSELRQR EGQRFGAAHV WENGARSNVT VRNTNQRLEP IRLRSTSNSR SRSPIQRQSG TVYHNSQRES RPVQQTTRRS VRRRGRTRVF LEQDRERERR GTAYTPFSNS RLVSRITVEE GEESSRSSTA VRRHPTITLD LQVRRIRPGE NRDRDSIANR TRSRVGLAEN TVTIESNSGG FRRTISRLER SGIRTYVSTI TVPLRRISEN ELVEPSSVAL RSILRQIMTG FGELSSLMEA DSESELQRNG QHLPDMHSEL SNLGTDNNRS QHREGSSQDR QAQGDSTEMH GENETTQPHT RNSDSRGGRQ LRNPNNLVET GTLPILRLAH FFLLNESDDD DRIRGLTKEQ IDNLSTRHYE HNSIDSELGK ICSVCISDYV TGNKLRQLPC MHEFHIHCID RWLSENCTCP ICRQPVLGSN IANNG |
| 分子量 | 78.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNF6蛋白的虚构参考文献示例：
1. **文献名称**：*RNF6 Promotes Prostate Cancer Progression by Ubiquitinating Androgen Receptor*
**作者**：Zhang Y. et al.
**摘要**：研究发现RNF6通过泛素化修饰雄激素受体(AR)，增强其稳定性并促进前列腺癌细胞增殖和转移，揭示了其在激素依赖性肿瘤中的关键作用。
2. **文献名称**：*Structural Insights into the E3 Ligase Activity of RNF6*
**作者**：Chen L. et al.
**摘要**：通过解析RNF6蛋白的晶体结构，阐明其RING结构域与泛素结合酶(E2)的相互作用机制，为靶向RNF6的抑制剂开发提供结构基础。
3. **文献名称**：*RNF6 Regulates Neuronal Differentiation via Ubiquitin-Proteasome Pathway*
**作者**：Wang X. et al.
**摘要**：实验证实RNF6通过泛素化降解特定转录抑制因子，激活神经分化相关基因表达，推动小鼠胚胎干细胞向神经元定向分化。
4. **文献名称**：*RNF6 Deficiency Induces Mitochondrial Dysfunction in Cardiomyocytes*
**作者**：Liu J. et al.
**摘要**：敲除RNF6导致心肌细胞线粒体分裂异常，引发氧化应激和细胞凋亡，提示其在心脏病理生理中的潜在调控作用。
(注：以上文献为模拟示例，实际研究中请参考具体数据库验证。)
RNF6 (Ring Finger Protein 6) is a member of the RING (Really Interesting New Gene) finger family of E3 ubiquitin ligases, which play pivotal roles in post-translational protein modification via the ubiquitin-proteasome system. Characterized by a conserved RING domain at its N-terminus, RNF6 facilitates the transfer of ubiquitin molecules to target proteins, marking them for proteasomal degradation or modulating their activity, localization, or interactions. The C-terminal region of RNF6 contains a unique acidic domain, which may influence substrate specificity or binding partners.
Functionally, RNF6 is implicated in diverse biological processes, including cell proliferation, apoptosis, and signal transduction. It has garnered significant attention for its role in cancer biology. Studies reveal that RNF6 is frequently overexpressed in malignancies such as prostate, breast, and colorectal cancers, where it promotes tumorigenesis by destabilizing tumor suppressors (e.g., AKTIP) or activating oncogenic pathways like the androgen receptor (AR) signaling in prostate cancer. Additionally, RNF6 regulates neural development and spermatogenesis, highlighting its tissue-specific functions. Despite these advances, the full spectrum of RNF6 substrates and mechanistic details of its context-dependent roles remain incompletely understood. Its dual potential as a therapeutic target or diagnostic biomarker in cancer continues to drive research, though challenges in targeting E3 ligases selectively underscore the need for deeper exploration of RNF6-related pathways.
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