| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF32 |
| Uniprot No | Q9H0A6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362 aa |
| 活性数据 | MLKNKGHSSK KDNLAVNAVA LQDHILHDLQ LRNLSVADHS KTQVQKKENK SLKRDTKAII DTGLKKTTQC PKLEDSEKEY VLDPKPPPLT LAQKLGLIGP PPPPLSSDEW EKVKQRSLLQ GDSVQPCPIC KEEFELRPQV LLSCSHVFHK ACLQAFEKFT NKKTCPLCRK NQYQTRVIHD GARLFRIKCV TRIQAYWRGC VVRKWYRNLR KTVPPTDAKL RKKFFEKKFT EISHRILCSY NTNIEELFAE IDQCLAINRS VLQQLEEKCG HEITEEEWEK IQVQALRRET HECSICLAPL SAAGGQRVGA GRRSREMALL SCSHVFHHAC LLALEEFSVG DRPPFHACPL CRSCYQKKIL EC |
| 分子量 | 41.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNF32蛋白的参考文献示例(注：以下内容为模拟生成，仅供参考)：
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1. **文献名称**: *"Cloning and Functional Analysis of Recombinant Human RNF32 as an E3 Ubiquitin Ligase"*
**作者**: Smith J, Brown K, Lee M
**摘要**: 本研究成功构建了重组人RNF32蛋白，并在HEK293细胞中表达纯化。实验证实RNF32通过其Ring结构域发挥E3泛素连接酶活性，并调控Wnt/β-catenin信号通路，提示其在胚胎发育中的作用。
2. **文献名称**: *"RNF32-Mediated Ubiquitination in Neural Crest Cell Migration"*
**作者**: Zhang L, Wang H, Ito T
**摘要**: 通过重组RNF32蛋白功能实验，发现其通过泛素化修饰转录因子Sox9.调控神经嵴细胞迁移，为神经管缺陷的分子机制提供新见解。
3. **文献名称**: *"Structural Characterization of Recombinant RNF32 and Its Role in ER Stress Response"*
**作者**: Garcia R, Müller S, Kim D
**摘要**: 解析了重组RNF32的晶体结构，揭示其与内质网应激蛋白IRE1α的相互作用，表明RNF32可能通过降解IRE1α负调控未折叠蛋白反应(UPR)。
4. **文献名称**: *"RNF32 Promotes Cancer Cell Invasion via Ubiquitin-Dependent TGF-β Pathway Activation"*
**作者**: Chen X, Zhou Y, Liu Q
**摘要**: 利用重组RNF32蛋白，研究证明其通过泛素化Smad7增强TGF-β信号通路，促进乳腺癌细胞侵袭，提示其作为潜在治疗靶点。
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**注**：以上文献及内容为示例，实际引用时需查询真实数据库(如PubMed、Google Scholar)获取准确信息。
**Background of Recombinant Human RNF32 Protein**
Recombinant human RNF32 (RING finger protein 32) is a ubiquitously expressed E3 ubiquitin ligase belonging to the RING finger protein family, characterized by a conserved C3HC4-type zinc-binding RING domain. This domain facilitates interaction with E2 ubiquitin-conjugating enzymes, enabling substrate-specific ubiquitination—a post-translational modification critical for regulating protein stability, localization, and activity. RNF32 is implicated in diverse cellular processes, including embryonic development, cell differentiation, and apoptosis. Studies suggest its involvement in neural tube formation and neurogenesis, with knockout models in mice showing developmental defects in the nervous system.
RNF32’s biological role extends to modulating signaling pathways such as Wnt and Notch, which are essential for tissue homeostasis and stem cell maintenance. Dysregulation of RNF32 has been linked to pathologies, including neurodegenerative diseases and cancers, though mechanistic details remain under investigation. The recombinant form of RNF32 is typically produced in mammalian or bacterial expression systems, allowing in vitro studies of its enzymatic activity, substrate binding, and interaction networks. Its application spans basic research exploring ubiquitin-proteasome system dynamics and drug discovery efforts targeting E3 ligases for therapeutic intervention. As an engineered protein, recombinant RNF32 retains functional fidelity, offering a tool to dissect its physiological and pathophysiological roles.
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