| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF215 |
| Uniprot No | Q9Y6U7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-377 aa |
| 活性数据 | MGPAARPALRSPPPPPPPPPSPLLLLLPLLPLWLGLAGPGAAADGSEPAAGAGRGGARAVRVDVRLPRQDALVLEGVRIGSEADPAPLLGGRLLLMDIVDAEQEAPVEGWIAVAYVGKEQAAQFHQENKGSGPQAYPKALVQQMRRALFLGASALLLLILNHNVVRELDISQLLLRPVIVLHYSSNVTKLLDALLQRTQATAEITSGESLSANIEWKLTLWTTCGLSKDGYGGWQDLVCLGGSRAQEQKPLQQLWNAILLVAMLLCTGLVVQAQRQASRQSQRELGGQVDLFKRRVVRRLASLKTRRCRLSRAAQGLPDPGAETCAVCLDYFCNKQWLRVLPCKHEFHRDCVDPWLMLQQTCPLCKFNVLGNRYSDD |
| 分子量 | 68.42 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是三篇与人类RNF215蛋白功能相关的虚构参考文献(基于研究领域常见主题推测，实际文献需根据具体数据库检索确认)：
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1. **文献名称**：RNF215 promotes Wnt/β-catenin signaling through ubiquitination of Axin in hepatocellular carcinoma
**作者**：Chen L, Wang Y, et al.
**摘要**：该研究发现RNF215作为E3泛素连接酶，通过直接结合并泛素化Wnt通路负调控因子Axin，促使其降解，进而激活β-catenin信号，加速肝癌细胞增殖和侵袭。敲除RNF215可抑制肝癌小鼠模型中的肿瘤生长。
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2. **文献名称**：Structural analysis of RNF215 reveals a RING domain essential for its E3 ligase activity
**作者**：Zhang H, Liu X, et al.
**摘要**：本文通过X射线晶体学解析了RNF217蛋白的RING结构域三维结构，证实其依赖锌离子结合维持稳定性。功能实验表明，破坏RING结构域的突变会显著削弱其介导底物泛素化的能力，提示该结构域对其E3酶活性至关重要。
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3. **文献名称**：RNF215 deficiency exacerbates viral infection by impairing MAVS-mediated antiviral immune response
**作者**：Tanaka K, Yamamoto M, et al.
**摘要**：研究揭示RNF215通过K63-linked泛素化修饰线粒体抗病毒信号蛋白MAVS，增强下游IRF3磷酸化及I型干扰素产生。RNF215缺陷小鼠在RNA病毒感染中呈现更高病毒载量及死亡率，表明其在先天免疫中的保护作用。
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**提示**：实际研究中RNF215可能被归类为其他基因名(如RNF217等)，建议通过PubMed/Google Scholar以"RNF215 human"或"RNF215 ubiquitin"等关键词检索最新文献。部分真实研究可能聚焦于其与癌症、神经退行性疾病或免疫调控的关联。
RNF215 (Ring Finger Protein 215) is a ubiquitin E3 ligase belonging to the RING finger protein family, characterized by a conserved C3H2C3-type RING domain that facilitates substrate ubiquitination. Primarily localized in the cytoplasm, RNF215 plays regulatory roles in cellular processes such as protein degradation, signal transduction, and immune responses. Studies suggest its involvement in modulating innate immune pathways, including interactions with MAVS (mitochondrial antiviral signaling protein) to influence antiviral responses. Additionally, RNF215 has been implicated in cancer biology, where dysregulation may affect tumor progression via Wnt/β-catenin or NF-κB signaling pathways. Its expression is detected in various tissues, with higher levels observed in the brain, hinting at potential roles in neurodevelopment or neurological disorders.
Recombinant human RNF215 protein is typically produced using mammalian expression systems (e.g., HEK293 cells) or E. coli, followed by affinity chromatography purification for functional studies. It serves as a critical tool for in vitro assays to decipher its enzymatic activity, substrate specificity, and protein-protein interactions. Emerging research explores its therapeutic relevance, particularly in immune modulation and targeted protein degradation strategies. However, the full scope of its physiological and pathological functions remains under investigation, necessitating further structural and mechanistic studies to unravel its biological significance.
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