| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF2 |
| Uniprot No | Q99496 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-336 aa |
| 活性数据 | SQAVQTNGT QPLSKTWELS LYELQRTPQE AITDGLEIVV SPRSLHSELM CPICLDMLKN TMTTKECLHR FCADCIITAL RSGNKECPTC RKKLVSKRSL RPDPNFDALI SKIYPSRDEY EAHQERVLAR INKHNNQQAL SHSIEEGLKI QAMNRLQRGK KQQIENGSGA EDNGDSSHCS NASTHSNQEA GPSNKRTKTS DDSGLELDNN NAAMAIDPVM DGASEIELVF RPHPTLMEKD DSAQTRYIKT SGNATVDHLS KYLAVRLALE ELRSKGESNQ MNLDTASEKQ YTIYIATASG QFTVLNGSFS LELVSEKYWK VNKPMELYYA PTKEHK |
| 分子量 | 37.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RNF2蛋白的参考文献示例(注：以下内容为虚构示例，仅供参考格式)：
1. **文献名称**: "RNF2 mediates histone H2A ubiquitination in Polycomb gene silencing"
**作者**: Wang et al.
**摘要**: 本研究揭示了重组人RNF2蛋白作为PRC1复合体的核心组分，通过体外泛素化实验证实其催化组蛋白H2A的K119位点泛素化，调控靶基因的转录沉默。
2. **文献名称**: "Expression and purification of recombinant human RNF2 in E. coli"
**作者**: Lee et al.
**摘要**: 文章报道了通过大肠杆菌系统高效表达可溶性RNF2蛋白的优化方法，并利用Ni-NTA层析纯化获得高纯度蛋白，为后续功能研究提供材料基础。
3. **文献名称**: "RNF2 promotes tumorigenesis by stabilizing c-Myc in hepatocellular carcinoma"
**作者**: Zhang et al.
**摘要**: 研究发现重组RNF2蛋白通过泛素化降解途径调控c-Myc蛋白稳定性，促进肝癌细胞增殖，为靶向RNF2的癌症治疗提供理论依据。
4. **文献名称**: "Structural analysis of RNF2-RING domain in ubiquitin ligase activity"
**作者**: Smith et al.
**摘要**: 通过X射线晶体学解析RNF2的RING结构域三维结构，结合突变实验验证其与E2泛素结合酶的作用界面，阐明其催化泛素转移的分子机制。
*注：以上文献及作者为假设案例，实际引用时需检索真实数据库(如PubMed)。*
RNF2 (RING Finger Protein 2), also known as RING1B, is a member of the Polycomb group (PcG) proteins, which play a critical role in epigenetic regulation of gene expression. It contains a characteristic RING finger domain that confers E3 ubiquitin-protein ligase activity. As a core component of the Polycomb Repressive Complex 1 (PRC1), RNF2 catalyzes the monoubiquitination of histone H2A at lysine 119 (H2AK119ub), a hallmark of transcriptionally repressive chromatin. This modification facilitates chromatin compaction and stable gene silencing, particularly in developmental processes, stem cell maintenance, and cellular differentiation. RNF2 works in coordination with other PRC1 subunits to establish long-term gene repression, often interacting with PRC2-mediated H3K27me3 marks for combinatorial epigenetic control. Dysregulation of RNF2 has been implicated in various cancers, where its overexpression correlates with tumor progression, metastasis, and poor prognosis. Studies using recombinant human RNF2 protein have been essential for dissecting its enzymatic mechanisms, structural features, and interactions with chromatin or partner proteins. Recombinant forms are widely employed in biochemical assays, drug discovery targeting epigenetic pathways, and functional studies exploring its role in cellular identity and disease pathogenesis. Its conserved function across species underscores its fundamental importance in chromatin biology.
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