| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF36 |
| Uniprot No | Q86WT6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341 aa |
| 活性数据 | MEEELAIQQGQLETTLKELQTLRNMQKEAIAAHKENKLHLQQHVSMEFLKLHQFLHSKEKDILTELREEGKALNEEMELNLSQLQEQCLLAKDMLVSIQAKTEQQNSFDFLKDITTLLHSLEQGMKVLATRELISRKLNLGQYKGPIQYMVWREMQDTLCPGLSPLTLDPKTAHPNLVLSKSQTSVWHGDIKKIMPDDPERFDSSVAVLGSRGFTSGKWYWEVEVAKKTKWTVGVVRESIIRKGSCPLTPEQGFWLLRLRNQTDLKALDLPSFSLTLTNNLDKVGIYLDYEGGQLSFYNAKTMTHIYTFSNTFMEKLYPYFCPCLNDGGENKEPLHILHPQ |
| 分子量 | 65.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于真实文献资料整理的关于RNF36(环指蛋白36)的3条参考文献示例(**注：RNF36的研究较少，以下信息为模拟概括，具体文献需结合实际情况查询验证**)：
1. **标题**：*RNF36 regulates apoptosis and cell cycle via ubiquitination in human cancer cells*
**作者**：Chen L, et al.
**摘要**：研究发现RNF36通过泛素化修饰调控促凋亡蛋白Bax的稳定性，重组RNF36蛋白在肺癌细胞中诱导caspase依赖性凋亡并阻滞细胞周期，提示其可能的肿瘤抑制功能。
2. **标题**：*Molecular cloning and characterization of human RNF36 as a testis-specific E3 ubiquitin ligase*
**作者**：Tanaka H, et al.
**摘要**：首次克隆并鉴定了人源RNF36基因，发现其特异性高表达于睾丸组织，重组蛋白体外实验显示其具有E3泛素连接酶活性，可能与精子发生相关。
3. **标题**：*RNF36 interacts with HSP70 and regulates protein quality control pathways*
**作者**：Wang Y, et al.
**摘要**：通过质谱分析发现RNF36与分子伴侣HSP70互作，重组RNF36蛋白的过表达通过泛素化错误折叠蛋白，增强细胞应激耐受性，提示其在蛋白质质量控制中的作用。
**备注**：RNF36的已知研究较少，上述内容为假设性示例。建议通过**PubMed/Google Scholar**搜索关键词“RNF36”或“Ring Finger Protein 36”获取最新文献。如有拼写误差(如可能为RNF43或RNF168等)，需进一步核实。
RNF36 (Ring Finger Protein 36), also known as HERC3. is a member of the HERC family of ubiquitin ligases characterized by a conserved RING finger domain and HECT-like regions. This protein plays a role in the ubiquitination process, a post-translational modification critical for regulating protein degradation, trafficking, and signaling. As an E3 ubiquitin ligase, RNF36 facilitates the transfer of ubiquitin molecules to specific substrate proteins, marking them for proteasomal degradation or functional modulation. Structurally, it contains a RING domain essential for catalytic activity and HERC motifs involved in substrate recognition. Although less studied than other HERC family members, RNF36 has been implicated in cellular processes such as DNA repair, cell cycle regulation, and immune response. Emerging research links it to diseases like cancer, where dysregulated ubiquitination contributes to tumor progression or suppression. Recombinant RNF36 protein, produced via expression systems like *E. coli* or mammalian cells, enables functional studies to elucidate its biological roles, substrate specificity, and interaction networks. Current challenges include clarifying its precise physiological targets and mechanisms in health and disease, highlighting its potential as a therapeutic target or biomarker.
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