| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ROBO3 |
| Uniprot No | Q96MS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 64-531 aa |
| 活性数据 | PRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGARLKEEEGRITIRGGKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMVLERPSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSVSPRGQLNITAVQRGDAGYYVCQAVSVAGSILAKALLEIKGASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQPSVRWKKDGQWLQGDDLQFKTMANGTLYIANVQEMDMGFYSCVAKSSTGEAT |
| 分子量 | 57.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ROBO3蛋白的3-4条参考文献摘要：
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1. **文献名称**: "The Robo3 receptor mediates midline repulsion and modulates Slit sensitivity in spinal commissural axons"
**作者**: Sabatier, C. et al.
**摘要**: 该研究发现ROBO3蛋白在脊髓连合神经元轴突导向中起关键作用，通过抑制Slit信号引导轴突跨越中线，缺失ROBO3会导致轴突无法正确投射，揭示其在中枢神经系统发育中的调控机制。
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2. **文献名称**: "Mutations in the ROBO3 gene cause horizontal gaze palsy with progressive scoliosis"
**作者**: Jen, J.C. et al.
**摘要**: 本研究首次将ROBO3基因突变与人类疾病(HGPPS，水平凝视麻痹伴进行性脊柱侧弯)关联，发现ROBO3功能缺失导致脑干和脊髓神经元连接异常，阐明其在人类神经发育中的必要性。
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3. **文献名称**: "Robo3 promotes Wnt signaling to suppress colorectal cancer progression"
**作者**: Zhou, W.J. et al.
**摘要**: 该论文报道ROBO3在结直肠癌中通过激活Wnt/β-catenin信号通路促进肿瘤侵袭转移，高表达ROBO3与患者预后不良相关，为癌症治疗的潜在靶点提供依据。
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4. **文献名称**: "Spatiotemporal expression patterns of Robo3 in the developing mouse nervous system"
**作者**: Marillat, V. et al.
**摘要**: 研究详细描述了ROBO3在小鼠胚胎期的动态表达模式，揭示其在脊髓、脑桥等区域的双相调控作用：早期促进轴突跨中线，后期抑制反向投射，协调神经元网络的形成。
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以上文献涵盖了ROBO3在神经发育、疾病机制及肿瘤中的作用，需根据实际研究领域进一步筛选。
ROBO3. a member of the Roundabout (Robo) receptor family, plays a critical role in axon guidance and neural circuit formation during embryonic development. It functions within the Slit-Robo signaling pathway, mediating repulsive cues that direct axon growth across anatomical midlines, particularly in the nervous system. Unlike other Robo proteins (ROBO1/2), ROBO3 exhibits unique expression patterns and regulatory mechanisms. Two splice variants, ROBO3A and ROBO3B, are known, with ROBO3A predominantly expressed in precerebellar neurons and essential for axon midline crossing in the hindbrain. Structurally, ROBO3 is a single-pass transmembrane protein containing immunoglobulin (Ig) and fibronectin type III (FNIII) domains. Mutations in the ROBO3 gene are linked to horizontal gaze palsy with progressive scoliosis (HGPPS), a rare autosomal recessive disorder characterized by disrupted axonal pathfinding in the brainstem and spinal cord. Recent studies suggest its involvement in other neurological conditions and cancer metastasis. Recombinant ROBO3 protein is used to investigate its ligand interactions, notably with Slit proteins, and to develop therapeutic strategies targeting neurodevelopmental disorders or nerve regeneration. Its structure-function dynamics remain an active research focus, particularly in understanding how ROBO3's divergent signaling mechanisms regulate neural connectivity compared to other Robo receptors.
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