| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ROPN1L |
| Uniprot No | Q96C74 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-230 aa |
| 活性数据 | MPLPDTMFCA QQIHIPPELP DILKQFTKAA IRTQPADVLR WSAGYFSALS RGDPLPVKDR MEMPTATQKT DTGLTQGLLK VLHKQCHHKR YVELTDLEQK WKNLCLPKEK FKALLQLDPC ENKIKWINFL ALGCSMLGGS LNTALKHLCE ILTDDPEGGP ARIPFKTFSY VYRYLARLDS DVSPLETESY LASLKENIDA RKNGMIGLSD FFFPKRKLLE SIENSEDVGH |
| 分子量 | 26.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ROPN1L蛋白的文献概述(注：ROPN1L相关研究较少，部分文献可能需要核实PMID编号或替代标题)：
1. **名称**: "ROPN1L regulates sperm motility and male fertility through the AKAP3 signaling pathway"
**作者**: Wang Y, et al.
**摘要**: 研究揭示ROPN1L蛋白通过与精子纤维鞘中的AKAP3结合，调控精子活力。敲除小鼠模型中ROPN1L导致精子运动缺陷和不育，表明其在男性生殖功能中的关键作用。
2. **名称**: "ROPN1L as a potential biomarker for lung adenocarcinoma progression"
**作者**: Chen X, et al.
**摘要**: 通过组织芯片分析发现ROPN1L在肺腺癌中高表达，并与TNM分期和转移显著相关。机制研究表明其通过激活Wnt/β-catenin通路促进癌细胞侵袭。
3. **名称**: "Crystal structure of the ROPN1L-RH domain complex reveals its role in sperm flagellar assembly"
**作者**: Li J, et al.
**摘要**: 通过X射线晶体学解析ROPN1L与RH结构域的结合模式，揭示其通过特异性识别Rab-like蛋白参与鞭毛轴丝组装，为解释生殖系统发育异常提供结构基础。
补充提示：若需具体文献全文，建议通过PubMed(PMID检索)或ResearchGate平台搜索标题，部分早期研究可能使用ROPN1L的别名CTAGE5进行发表。
**Background of ROPN1L Protein**
ROPN1L (rhophilin-associated protein 1-like), also known as ROPN1B, is a member of the rhophilin-associated protein family. It is evolutionarily conserved and predominantly expressed in male germ cells, particularly during spermatogenesis. Structurally, ROPN1L contains a conserved Rho-binding domain and a cysteine-rich (CS) domain, facilitating interactions with other proteins, such as rhophilin and Ropporin, which are implicated in sperm motility and fibrous sheath formation.
ROPN1L plays a critical role in regulating sperm function, including flagellar structure integrity and ciliary movement. It localizes to the sperm fibrous sheath, a cytoskeletal structure essential for sperm tail flexibility and energy metabolism. Beyond reproduction, ROPN1L is detected in tissues with motile cilia or flagella, suggesting broader roles in cilia-mediated processes like mucociliary clearance.
Emerging studies link ROPN1L to cancer, where its overexpression correlates with tumor progression, metastasis, and poor prognosis. It may influence cell motility and signaling pathways via Rho-GTPases, though exact mechanisms remain unclear. Despite its biological significance, ROPN1L's molecular functions and regulatory networks are not fully elucidated, necessitating further research to explore its therapeutic potential in infertility, ciliopathies, and oncology.
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