| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RTCD1 |
| Uniprot No | O00442 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-366 aa |
| 活性数据 | MAGPRVEVDG SIMEGGGQIL RVSTALSCLL GLPLRVQKIR AGRSTPGLRP QHLSGLEMIR DLCDGQLEGA EIGSTEITFT PEKIKGGIHT ADTKTAGSVC LLMQVSMPCV LFAASPSELH LKGGTNAEMA PQIDYTVMVF KPIVEKFGFI FNCDIKTRGY YPKGGGEVIV RMSPVKQLNP INLTERGCVT KIYGRAFVAG VLPFKVAKDM AAAAVRCIRK EIRDLYVNIQ PVQEPKDQAF GNGNGIIIIA ETSTGCLFAG SSLGKRGVNA DKVGIEAAEM LLANLRHGGT VDEYLQDQLI VFMALANGVS RIKTGPVTLH TQTAIHFAEQ IAKAKFIVKK SEDEEDAAKD TYIIECQGIG MTNPNL |
| 分子量 | 39.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人RTCD1蛋白的3篇示例文献(注：RTCD1为虚构缩写，实际文献需通过学术数据库查询。以下内容仅为示例格式参考)：
1. **文献名称**: *Recombinant Human RTCD1 Induces Apoptosis in Colorectal Cancer Cells*
**作者**: Smith J. et al.
**摘要**: 研究重组RTCD1蛋白通过激活线粒体途径诱导结直肠癌细胞凋亡的机制，证实其在体外抑制肿瘤细胞增殖的作用。
2. **文献名称**: *Structural Characterization of RTCD1 and Its Interaction with TNF-α Signaling Pathway*
**作者**: Zhang L. et al.
**摘要**: 通过X射线晶体学解析RTCD1蛋白的三维结构，并揭示其通过与TNF-α受体结合调控炎症反应的功能。
3. **文献名称**: *RTCD1 Recombinant Protein Attenuates Neuronal Oxidative Stress in Alzheimer’s Disease Models*
**作者**: Kumar R. et al.
**摘要**: 证明重组RTCD1蛋白通过减少Aβ诱导的氧化应激，改善阿尔茨海默病模型中的神经元存活率。
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如需真实文献，建议在PubMed或Web of Science搜索**“recombinant RTCD1”**或结合其功能关键词(如凋亡/结构/疾病模型)进行筛选。
Recombinant human RTCD1 (RNA 3'-terminal phosphate cyclase-like protein) is a conserved enzyme involved in RNA processing and repair. It belongs to the RNA cyclase family, characterized by a catalytic cyclase domain that facilitates RNA ligation through a unique mechanism involving autoubgiquitination-like intermediates. RTCD1 plays a critical role in the unfolded protein response (UPR) by mediating the non-conventional splicing of XBP1 mRNA during endoplasmic reticulum stress, thereby regulating genes responsible for protein folding and degradation.
Originally identified for its tRNA repair functions in bacteria and archaea, the mammalian homolog RTCD1 has gained attention for its dual roles in RNA quality control and stress signaling. Structurally, it forms a dynamic homodimer and interacts with components of the UPR pathway, linking RNA metabolism to cellular stress adaptation. Dysregulation of RTCD1 activity is associated with neurodegenerative diseases, cancer progression, and immune disorders due to its impact on stress-induced apoptosis and inflammation.
Recombinant RTCD1 protein is typically produced in E. coli or mammalian expression systems for biochemical studies, structural analysis, and drug discovery targeting UPR-related pathologies. Its applications span investigating RNA repair mechanisms, developing therapeutic strategies for protein-misfolding diseases, and exploring novel biomarkers for cellular stress responses.
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