| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RSC1A1 |
| Uniprot No | Q92681 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-617 aa |
| 活性数据 | MSSLPTSDGF NHPARSSGQS PDVGNPMSLA RSVSASVCPI KPSDSDRIEP KAVKALKASA EFQLNSEKKE HLSLQDLSDH ASSADHAPTD QSPAMPMQNS SEEITVAGNL EKSAERSTQG LKFHLHTRQE ASLSVTSTRM HEPQMFLGEK DWHPENQNLS QVSDPQQHEE PGNEQYEVAQ QKASHDQEYL CNIGDLELPE ERQQNQHKIV DLEATMKGNG LPQNVDPPSA KKSIPSSECS GCSNSETFME IDTAQQSLVT LLNSTGRQNA NVKNIGALDL TLDNPLMEVE TSKCNPSSEI LNDSISTQDL QPPETNVEIP GTNKEYGHYS SPSLCGSCQP SVESAEESCP SITAALKELH ELLVVSSKPA SENTSEEVIC QSETIAEGQT SIKDLSERWT QNEHLTQNEQ CPQVSFHQAI SVSVETEKLT GTSSDTGREA VENVNFRSLG DGLSTDKEGV PKSRESINKN RSVTVTSAKT SNQLHCTLGV EISPKLLAGE EDALNQTSEQ TKSLSSNFIL VKDLGQGIQN SVTDRPETRE NVCPDASRPL LEYEPPTSHP SSSPAILPPL IFPATDIDRI LRAGFTLQEA LGALHRVGGN ADLALLVLLA KNIVVPT |
| 分子量 | 66.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RSC1A1(SLC32A1/VGAT)蛋白的模拟参考文献示例(仅供参考，非真实文献)：
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1. **标题**：*Expression and functional characterization of recombinant human RSC1A1 in Xenopus oocytes*
**作者**：Schmidt et al. (2010)
**摘要**：研究报道了人源RSC1A1蛋白在非洲爪蟾卵母细胞中的重组表达及功能分析，揭示了其在γ-氨基丁酸(GABA)转运中的pH依赖性特性，为神经递质转运机制提供了新见解。
2. **标题**：*Structural insights into the vesicular inhibitory amino acid transporter (VGAT/RSC1A1) by cryo-EM*
**作者**：Li et al. (2018)
**摘要**：通过冷冻电镜解析了重组人RSC1A1蛋白的三维结构，阐明其转运抑制性神经递质(如GABA)的分子机制，并发现潜在药物结合位点。
3. **标题**：*RSC1A1-mediated vesicular transport in cancer cell metabolism*
**作者**：Gomez et al. (2021)
**摘要**：探讨了重组RSC1A1在肿瘤细胞中的异常表达如何通过调控氨基酸转运促进代谢重编程，为靶向癌症代谢的治疗策略提供依据。
4. **标题**：*High-yield purification of recombinant SLC32A1 for biochemical assays*
**作者**：Park & Kim (2015)
**摘要**：开发了一种基于昆虫细胞表达系统的高效重组RSC1A1纯化方法，验证其在体外跨膜转运活性，优化了药物筛选平台。
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**备注**：以上为人工模拟的参考文献，实际研究中建议通过 **PubMed/Google Scholar** 检索真实文献(关键词如“RSC1A1 recombinant”“SLC32A1 expression”)。
**Background of Recombinant Human SLC1A1 (RSC1A1) Protein**
The recombinant human solute carrier family 1 member 1 (SLC1A1), also known as excitatory amino acid transporter 3 (EAAT3) or EAAC1. is a sodium-dependent glutamate transporter integral to cellular amino acid homeostasis. Predominantly expressed in neurons, kidney proximal tubules, and intestinal epithelial cells, SLC1A1 facilitates the uptake of glutamate and aspartate, crucial for neurotransmission, metabolic regulation, and antioxidant defense via cysteine transport. Structurally, it features ten transmembrane domains with intracellular N- and C-termini, functioning through a conserved mechanism involving conformational shifts during substrate translocation.
Dysregulation of SLC1A1 is linked to neurological disorders (e.g., epilepsy, schizophrenia) and renal diseases. Recombinant SLC1A1. produced using expression systems like *E. coli* or mammalian cells, retains native functionality, enabling studies on transport kinetics, drug interactions, and disease mechanisms. Its applications span drug screening, structural biology (e.g., cryo-EM studies), and therapeutic development targeting glutamate-related pathologies. Additionally, SLC1A1’s role in mTOR signaling and oxidative stress highlights its broader impact on cellular health. Engineered variants (e.g., fluorescent-tagged or mutant forms) further aid in elucidating its physiological and pathological roles.
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