| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RSAD1 |
| Uniprot No | Q9HA92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-442 aa |
| 活性数据 | AQR RRRVENAGGS PSPEPAGRRA ALYVHWPYCE KRCSYCNFNK YIPRRLEEAA MQKCLVTEAQ TLLRLSGVQR VESVFFGGGT PSLASPHTVA AVLEAVAQAA HLPADLEVTL EANPTSAPGS RLAEFGAAGV NRLSIGLQSL DDTELRLLGR THSACDALRT LAEARRLFPG RVSVDLMLGL PAQQVGPWLG QLQELLHHCD DHLSLYQLSL ERGTALFAQV QRGALPAPDP ELAAEMYQRG RAVLREAGFH QYEVSNFARN GALSTHNWTY WQCGQYLGVG PGAHGRFMPQ GAGGHTREAR IQTLEPDNWM KEVMLFGHGT RKRVPLGRLE LLEEVLALGL RTDVGITHQH WQQFEPQLTL WDVFGANKEV QELLERGLLQ LDHRGLRCSW EGLAVLDSLL LTLLPQLQEA WQQRTPSPVP GG |
| 分子量 | 48.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人RSAD1蛋白的虚拟参考文献示例(请注意文献为模拟数据，非真实存在)：
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**1. 文献名称**: *Antiviral Role of RSAD1 in Interferon-Mediated Inhibition of Hepatitis C Virus Replication*
**作者**: Liu Y, et al.
**摘要**: 发现RSAD1是干扰素α诱导的抗病毒蛋白，通过靶向HCV RNA聚合酶抑制病毒复制，实验证明重组RSAD1体外表达可降低HCV核心蛋白水平。
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**2. 文献名称**: *Structural Characterization of Human RSAD1 and Its SAM-Dependent Enzymatic Mechanism*
**作者**: Zhang Q, Smith JL
**摘要**: 首次解析重组人RSAD1的晶体结构，揭示其依赖S-腺苷甲硫氨酸(SAM)的活性位点，酶活实验表明其通过自由基机制参与宿主先天免疫反应。
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**3. 文献名称**: *RSAD1 as a Biomarker for Influenza A Virus Susceptibility*
**作者**: Watanabe T, et al.
**摘要**: 通过基因敲除模型发现RSAD1敲除小鼠对甲型流感病毒易感性显著增加，重组RSAD1蛋白处理可恢复细胞抗病毒反应，提示其作为治疗靶点的潜力。
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提示：如需真实文献，可检索PubMed或SciHub数据库，关键词"RSAD1"或"viperin"(注：RSAD1又名viperin，为干扰素诱导的重要抗病毒蛋白)。
RSAD1 (Radical S-Adenosyl Methionine Domain-Containing Protein 1), also known as viperin or CIG5. is an interferon-inducible protein that plays a critical role in innate immunity against viral infections. First identified for its antiviral activity, RSAD1 is conserved across vertebrates and expressed in various tissues, particularly in immune cells. It functions by disrupting viral replication cycles through multiple mechanisms, including enzymatic activity linked to its radical S-adenosylmethionine (SAM) domain. This domain enables RSAD1 to catalyze radical-mediated reactions, producing antiviral molecules like ddhCTP, which inhibits viral RNA polymerases.
Structurally, RSAD1 contains an N-terminal amphipathic helix for membrane association, a central radical SAM domain, and a variable C-terminal region. Its expression is strongly upregulated by type I and III interferons, cytokines released during viral infections. Beyond antiviral defense, RSAD1 is implicated in modulating inflammation, lipid metabolism, and mitochondrial function. Studies link it to metabolic pathways, including interactions with mitochondrial enzymes involved in fatty acid oxidation and energy production.
Emerging research also associates RSAD1 with non-infectious diseases, such as cancer and metabolic disorders, highlighting its multifaceted roles in cellular homeostasis. Despite progress, its exact biochemical mechanisms and regulatory networks remain under investigation, making it a focus of both therapeutic and basic research.
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