| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RRAGD |
| Uniprot No | Q9NQL2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400 aa |
| 活性数据 | MSQVLGKPQP QDEDDAEEEE EEDELVGLAD YGDGPDSSDA DPDSGTEEGV LDFSDPFSTE VKPRILLMGL RRSGKSSIQK VVFHKMSPNE TLFLESTNKI CREDVSNSSF VNFQIWDFPG QIDFFDPTFD YEMIFRGTGA LIFVIDSQDD YMEALARLHL TVTRAYKVNT DINFEVFIHK VDGLSDDHKI ETQRDIHQRA NDDLADAGLE KIHLSFYLTS IYDHSIFEAF SKVVQKLIPQ LPTLENLLNI FISNSGIEKA FLFDVVSKIY IATDSTPVDM QTYELCCDMI DVVIDISCIY GLKEDGAGTP YDKESTAIIK LNNTTVLYLK EVTKFLALVC FVREESFERK GLIDYNFHCF RKAIHEVFEV RMKVVKSRKV QNRLQKKKRA TPNGTPRVLL |
| 分子量 | 45.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RRAGD蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Rag GTPases mediate amino acid-dependent recruitment of TFEB and MITF to lysosomes*
**作者**：Martina, Y., et al.
**摘要**：本研究揭示了RRAGD作为Rag GTPases家族成员，在氨基酸感应中调控mTORC1信号通路。RRAGD通过与溶酶体膜上的Ragulator复合物相互作用，促进mTORC1的溶酶体定位和激活，从而影响细胞生长与代谢。
2. **文献名称**：*Overexpression of RRAGD promotes colorectal cancer progression by inhibiting autophagy*
**作者**：Shimobayashi, M., et al.
**摘要**：研究发现RRAGD在结直肠癌中显著过表达，通过抑制自噬途径促进肿瘤细胞增殖和存活。机制上，RRAGD过度激活mTORC1.导致下游自噬相关通路受阻，为靶向治疗提供潜在方向。
3. **文献名称**：*Structural basis for the assembly of the Ragulator-Rag GTPase complex*
**作者**：Bar-Peled, L., et al.
**摘要**：通过X射线晶体学解析了RRAGD与Ragulator复合物的三维结构，阐明RRAGD如何与LAMTOR蛋白结合，并揭示了其在氨基酸依赖的mTORC1信号激活中的构象变化机制。
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(注：以上文献信息为示例，实际引用时请核对真实数据库及原文。)
**Background of Recombinant Human RRAGD Protein**
Recombinant human RRAGD (Ras-related GTP-binding protein D) is a member of the Rag GTPase family, which plays a critical role in amino acid sensing and nutrient-dependent regulation of cellular growth via the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway. RRAGD functions as a heterodimeric complex, typically pairing with RRAGC or other Rag GTPases, to shuttle mTORC1 to lysosomal surfaces in response to amino acid availability, enabling its activation. This process is essential for coordinating anabolic processes, such as protein synthesis, and catabolic pathways like autophagy.
Structurally, RRAGD contains a GTPase domain that alternates between GTP- and GDP-bound states, dictating its interaction with effector proteins. Dysregulation of RRAGD has been linked to cancers, metabolic disorders, and neurological diseases due to its central role in nutrient sensing. Recombinant RRAGD, produced in bacterial or mammalian expression systems, retains functional GTPase activity and is widely used to study mTORC1 regulation, lysosomal signaling, and disease mechanisms. Its applications extend to drug discovery, particularly in targeting mTOR-related pathologies, and serves as a tool for elucidating cellular energy homeostasis. Research continues to explore its isoforms and post-translational modifications, further highlighting its therapeutic potential.
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