| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPUSD1 |
| Uniprot No | Q9UJJ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312 aa |
| 活性数据 | MEPGSVENLS IVYRSRDFLV VNKHWDVRID SKAWRETLTL QKQLRYRFPE LADPDTCYGF RFCHQLDFST SGALCVALNK AAAGSAYRCF KERRVTKAYL ALLRGHIQES RVTISHAIGR NSTEGRAHTM CIEGSQGCEN PKPSLTDLVV LEHGLYAGDP VSKVLLKPLT GRTHQLRVHC SALGHPVVGD LTYGEVSGRE DRPFRMMLHA FYLRIPTDTE CVEVCTPDPF LPSLDACWSP HTLLQSLDQL VQALRATPDP DPEDRGPRPG SPSALLPGPG RPPPPPTKPP ETEAQRGPCL QWLSEWTLEP DS |
| 分子量 | 34.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **标题**："Structural Insights into the RNA Pseudouridylation Mechanism of Human RPUSD1"
**作者**：Chen L, et al.
**摘要**：该研究通过X射线晶体学解析了重组人RPUSD1蛋白的三维结构，揭示了其催化RNA假尿苷修饰的关键活性位点，并发现其依赖金属离子辅助的催化机制，为理解其在RNA代谢中的作用提供结构基础。
2. **标题**："Recombinant RPUSD1 Regulates Mitochondrial RNA Processing and Cellular Stress Response"
**作者**：Park S, et al.
**摘要**：通过在大肠杆菌中表达重组RPUSD1蛋白，研究发现其能特异性修饰线粒体RNA的假尿苷位点，调控氧化应激条件下细胞的存活能力，提示RPUSD1在应激反应中的潜在功能。
3. **标题**："Dysregulation of RPUSD1 in Hepatocellular Carcinoma: Implications for RNA Modification and Tumor Progression"
**作者**：Wang Y, et al.
**摘要**：该文利用重组RPUSD1蛋白进行体外功能实验，发现其在肝癌组织中表达异常，并通过改变靶RNA稳定性促进癌细胞增殖，表明其可能作为癌症治疗的潜在靶点。
4. **标题**："Biochemical Characterization of Recombinant Human RPUSD1: Substrate Specificity and Enzymatic Kinetics"
**作者**：Gonzalez R, et al.
**摘要**：研究通过体外酶活实验系统分析了重组RPUSD1对不同RNA底物的催化效率，发现其对特定序列具有偏好性，并揭示了其动力学参数，为开发相关抑制剂奠定基础。
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注：以上文献信息为示例性质，实际引用需查阅真实数据库(如PubMed)。如需真实文献，建议通过关键词检索获取。
Recombinant human RPUSD1 (RNA Pseudouridine Synthase Domain-Containing 1) protein is a biologically engineered variant of the naturally occurring enzyme encoded by the RPUSD1 gene. RPUSD1 belongs to the pseudouridine synthase family, which catalyzes the isomerization of uridine to pseudouridine (Ψ) in RNA molecules—a common post-transcriptional modification critical for RNA stability, structure, and function. This protein contains conserved catalytic domains responsible for its enzymatic activity, particularly in non-coding RNAs and possibly mRNA, though its specific substrate preferences remain under investigation.
RPUSD1 is implicated in cellular processes such as ribosome biogenesis, stress response, and gene expression regulation. Dysregulation of pseudouridylation has been linked to cancers, neurological disorders, and mitochondrial diseases, highlighting RPUSD1's potential role in pathogenesis. Recombinant RPUSD1 is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies, enabling researchers to explore its biochemical properties, interaction networks, and mechanisms in disease models.
Current research focuses on elucidating its structural motifs, substrate specificity, and functional crosstalk with other RNA-modifying enzymes. Understanding RPUSD1 could advance RNA epigenetics and therapeutic strategies targeting RNA modifications.
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