| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS8 |
| Uniprot No | P62241 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-208 aa |
| 活性数据 | GISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS LLEEQFQQGK LLACIASRPG QCGRADGYVL EGKELEFYLR KIKARKGK |
| 分子量 | 24.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条关于重组人RPS8蛋白的模拟参考文献(内容为示例，非真实文献)：
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1. **文献名称**: *Structural characterization of recombinant human RPS8 and its role in ribosome assembly*
**作者**: Zhang L, et al.
**摘要**: 通过X射线晶体学解析了重组人RPS8蛋白的三维结构，发现其C端结构域在核糖体小亚基组装中起关键作用，并提出了与其他核糖体蛋白相互作用的分子机制。
2. **文献名称**: *Functional analysis of RPS8 in mRNA translation fidelity*
**作者**: Kim S, et al.
**摘要**: 研究显示重组RPS8蛋白通过稳定18S rRNA的特定区域调控mRNA解码的准确性，敲低RPS8导致翻译错误率显著增加，提示其在蛋白质质量控制中的重要性。
3. **文献名称**: *Overexpression of recombinant RPS8 promotes cancer cell proliferation via mTOR signaling*
**作者**: Wang Y, et al.
**摘要**: 发现重组RPS8在多种癌细胞系中异常高表达，并通过激活mTOR通路加速细胞周期进程，表明其可能作为癌症治疗的潜在靶点。
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注：以上为示例，实际文献需通过学术数据库(PubMed、Web of Science等)检索确认。
The ribosomal protein S8 (RPS8) is a key component of the 40S ribosomal subunit, playing a critical role in ribosome biogenesis and protein synthesis. As part of the small ribosomal subunit, RPS8 contributes to the structural integrity of the ribosome and facilitates the accurate decoding of mRNA during translation. It interacts with rRNA and other ribosomal proteins to stabilize the ribosome’s architecture and ensure proper assembly. Beyond its canonical role in translation, RPS8 has been implicated in extraribosomal functions, including cell proliferation, apoptosis, and stress response, with dysregulation linked to cancers and genetic disorders.
Recombinant human RPS8 (rhRPS8) is produced via genetic engineering, often using expression systems like *E. coli* or mammalian cells, enabling high-purity protein for research. Its study aids in elucidating ribosome assembly mechanisms, translational fidelity, and disease pathogenesis. For instance, mutations in RPS8 have been associated with Diamond-Blackfan anemia and colorectal cancer, making it a potential biomarker or therapeutic target. Additionally, rhRPS8 serves as a tool for structural studies (e.g., cryo-EM) and exploring ribosomopathies. Research continues to uncover its regulatory roles in cellular processes, highlighting its significance in both basic biology and clinical applications.
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