| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS6KA2 |
| Uniprot No | Q15349 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-733 aa |
| 活性数据 | MDLSMKKFAV RRFFSVYLRR KSRSKSSSLS RLEEEGVVKE IDISHHVKEG FEKADPSQFE LLKVLGQGSY GKVFLVRKVK GSDAGQLYAM KVLKKATLKV RDRVRSKMER DILAEVNHPF IVKLHYAFQT EGKLYLILDF LRGGDLFTRL SKEVMFTEED VKFYLAELAL ALDHLHSLGI IYRDLKPENI LLDEEGHIKI TDFGLSKEAI DHDKRAYSFC GTIEYMAPEV VNRRGHTQSA DWWSFGVLMF EMLTGSLPFQ GKDRKETMAL ILKAKLGMPQ FLSGEAQSLL RALFKRNPCN RLGAGIDGVE EIKRHPFFVT IDWNTLYRKE IKPPFKPAVG RPEDTFHFDP EFTARTPTDS PGVPPSANAH HLFRGFSFVA SSLIQEPSQQ DLHKVPVHPI VQQLHGNNIH FTDGYEIKED IGVGSYSVCK RCVHKATDTE YAVKIIDKSK RDPSEEIEIL LRYGQHPNII TLKDVYDDGK FVYLVMELMR GGELLDRILR QRYFSEREAS DVLCTITKTM DYLHSQGVVH RDLKPSNILY RDESGSPESI RVCDFGFAKQ LRAGNGLLMT PCYTANFVAP EVLKRQGYDA ACDIWSLGIL LYTMLAGFTP FANGPDDTPE EILARIGSGK YALSGGNWDS ISDAAKDVVS KMLHVDPHQR LTAMQVLKHP WVVNREYLSP NQLSRQDVHL VKGAMAATYF ALNRTPQAPR LEPVLSSNLA QRRGMKRLTS TRL |
| 分子量 | 83.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于学术论文结构的示例参考文献(注：实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of Human RPS6KA2 in MAPK Signaling"*
**作者**: Zhang, Y. et al.
**摘要**: 研究报道了RPS6KA2蛋白在大肠杆菌系统中的重组表达及纯化，证实其激酶活性依赖于ERK1/2的磷酸化激活，并调控下游转录因子CREB的活性。
2. **文献名称**: *"Structural Insights into RPS6KA2 Substrate Specificity and Implications for Cancer Therapy"*
**作者**: Thompson, R.B. et al.
**摘要**: 通过X射线晶体学解析了重组RPS6KA2的激酶结构域，揭示了其与ATP类似物及底物肽段的结合模式，为靶向抑制剂设计提供了依据。
3. **文献名称**: *"RPS6KA2 Modulates Synaptic Plasticity via Phosphorylation of Neurological Targets"*
**作者**: Li, H. & Park, J.
**摘要**: 研究利用HEK293细胞表达重组RPS6KA2.发现其通过磷酸化AMPA受体亚基GluA1调控突触传递，提示其在神经退行性疾病中的潜在作用。
4. **文献名称**: *"Dysregulation of RPS6KA2 in Triple-Negative Breast Cancer and Its Association with Chemoresistance"*
**作者**: García-Sánchez, A. et al.
**摘要**: 通过体外重组蛋白实验及临床样本分析，证实RPS6KA2过表达通过激活mTOR通路促进乳腺癌细胞存活，并与化疗耐药性相关。
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**注意**：以上文献为示例性内容，实际研究中请通过 **PubMed**、**Web of Science** 或 **Google Scholar** 等平台检索真实文献，关键词可包括 **"RPS6KA2 recombinant"**、**"RSK3 signaling"** 等。
Ribosomal protein S6 kinase A2 (RPS6KA2), also known as p90 ribosomal S6 kinase 3 (RSK3), is a serine/threonine kinase belonging to the RSK family, which is downstream of the Ras/MAPK signaling pathway. It plays a critical role in translating extracellular signals into cellular responses, including proliferation, survival, and differentiation. Structurally, RPS6KA2 contains two functional kinase domains: an N-terminal kinase responsible for substrate phosphorylation and a C-terminal kinase regulated by ERK1/2-mediated activation.
This kinase is activated through sequential phosphorylation by MAPK/ERK and PDK1. enabling it to phosphorylate downstream targets such as transcription factors (e.g., CREB, NF-κB) and components of the translational machinery. RPS6KA2 is highly expressed in the brain, muscle, and endocrine tissues, suggesting roles in neuronal development, synaptic plasticity, and hormone signaling. Dysregulation of RPS6KA2 has been implicated in neurological disorders (e.g., autism spectrum disorders, intellectual disability) and cancers, where it may promote tumor growth or metastasis.
Recombinant RPS6KA2 protein, produced via bacterial or mammalian expression systems, is widely used to study its enzymatic activity, substrate interactions, and inhibitor screening for therapeutic development. Its modular structure and regulatory complexity make it a key target for understanding signal transduction mechanisms and disease pathology.
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