| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS26 |
| Uniprot No | P62854 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-115 aa |
| 活性数据 | TKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSRE ARKDRTPPPR FRPAGAAPRP PPKPM |
| 分子量 | 13.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPS26蛋白的参考文献示例(注：以下内容为假设性摘要，基于常见研究方向推断)：
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1. **文献名称**：*"Crystal structure of human RPS26 reveals ribosomal protein assembly dynamics"*
**作者**：Zhang Y, et al.
**摘要**：通过X射线晶体学解析了重组人RPS26的三维结构，揭示了其核糖体结合界面及与其他亚基的相互作用，表明RPS26在40S核糖体亚基组装中起关键作用。
2. **文献名称**：*"RPS26 deficiency disrupts mRNA translation and induces endoplasmic reticulum stress in human cells"*
**作者**：Li H, Wang T.
**摘要**：研究利用重组RPS26蛋白进行功能回补实验，发现RPS26缺失导致mRNA翻译精确性下降，并激活未折叠蛋白反应(UPR)，提示其与内质网应激相关疾病的潜在联系。
3. **文献名称**：*"Recombinant RPS26 as a potential biomarker for ribosomopathies screening"*
**作者**：Garcia-Sanchez A, et al.
**摘要**：建立基于重组人RPS26的ELISA检测方法，分析患者血清中RPS26抗体水平，为先天性核糖体病(如Diamond-Blackfan贫血)的筛查提供新策略。
4. **文献名称**：*"Functional analysis of RPS26 mutations in cancer using recombinant protein models"*
**作者**：Chen X, et al.
**摘要**：通过表达携带肿瘤相关突变的重组RPS26.发现特定突变体破坏核糖体与肿瘤抑制因子mRNA的结合，可能导致肿瘤发生中蛋白质翻译失调。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词 **"recombinant human RPS26"** 或 **"RPS26 protein function"** 获取最新研究。
**Background of Recombinant Human RPS26 Protein**
Ribosomal Protein S26 (RPS26) is a component of the 40S subunit of eukaryotic ribosomes, essential for mRNA translation. As part of the small ribosomal subunit, RPS26 contributes to ribosome assembly, stability, and the accurate decoding of genetic information. Structurally, it contains conserved domains that facilitate interactions with rRNA and other ribosomal proteins. Beyond its canonical role in protein synthesis, RPS26 has been implicated in extraribosomal functions, including cellular stress responses and apoptosis regulation.
Mutations in the RPS26 gene are linked to human diseases, notably Diamond-Blackfan anemia (DBA), a rare bone marrow disorder characterized by red blood cell deficiency. Additionally, dysregulation of RPS26 expression has been observed in certain cancers, suggesting potential roles in tumorigenesis. Studies using recombinant human RPS26 protein enable mechanistic explorations of these associations, including interactions with RNA or proteins, and functional assays in disease models.
Recombinant RPS26 is typically produced via bacterial or eukaryotic expression systems, followed by purification to homogeneity. Its availability supports structural studies (e.g., X-ray crystallography) and functional analyses, such as investigating ribosome biogenesis defects in DBA. Ongoing research aims to elucidate how RPS26 variants disrupt cellular homeostasis, offering insights into therapeutic strategies for ribosomal disorders.
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