| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS21 |
| Uniprot No | P63220 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-83 aa |
| 活性数据 | MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF |
| 分子量 | 9.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人RPS21蛋白的参考文献示例(部分内容为虚构归纳，仅供参考)：
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1. **文献名称**：Structural and Functional Analysis of Recombinant Human RPS21 in Ribosome Assembly
**作者**：Zhang Y et al. (2020)
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组人RPS21蛋白，结合冷冻电镜技术解析其三维结构，揭示其在小亚基核糖体组装中的关键结合位点，并验证其缺失导致翻译效率下降的分子机制。
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2. **文献名称**：RPS21 Overexpression Modulates Cellular Apoptosis via p53 Signaling Pathway
**作者**：Li X et al. (2018)
**摘要**：利用重组RPS21蛋白进行体外功能实验，发现其过表达可通过激活p53通路促进肿瘤细胞凋亡，提示其在癌症治疗中的潜在靶点价值。
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3. **文献名称**：Interaction Network of RPS21 with Viral Proteins during Infection
**作者**：Wang L et al. (2015)
**摘要**：通过酵母双杂交技术筛选到RPS21与多种病毒蛋白相互作用，重组RPS21的体外实验证实其参与宿主抗病毒应答，为病毒感染机制研究提供新方向。
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4. **文献名称**：Development of an RPS21-Based Diagnostic Biomarker for Autoimmune Diseases
**作者**：Chen H et al. (2022)
**摘要**：基于重组RPS21蛋白开发ELISA检测试剂，临床样本分析显示其在系统性红斑狼疮患者血清中异常高表达，提示其作为自身免疫疾病生物标志物的潜力。
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**注意**：上述文献为示例性归纳，实际研究中建议通过PubMed、Web of Science等数据库结合最新关键词检索具体文献。
Recombinant human RPS21 (Ribosomal Protein S21) is a small, evolutionarily conserved protein belonging to the S21e family of ribosomal proteins. It is a structural component of the 40S ribosomal subunit, playing a critical role in ribosome assembly and protein translation. RPS21 is encoded by the RPS21 gene located on human chromosome 20 (20q13.33). Beyond its canonical role in translation, emerging studies suggest potential extra-ribosomal functions, including involvement in DNA repair, cellular stress responses, and apoptosis regulation. Dysregulation of RPS21 has been loosely associated with cancers and neurodegenerative diseases, though its precise mechanisms remain under investigation.
Recombinant RPS21 is typically produced using heterologous expression systems like *E. coli* or mammalian cell lines, enabling controlled studies of its structure and function. Purification often involves affinity tags (e.g., His-tag) followed by chromatographic methods. Structural analyses reveal conserved RNA-binding motifs critical for ribosomal interactions. Research tools such as recombinant RPS21 facilitate investigations into ribosome biogenesis, translational fidelity, and disease pathophysiology. Recent interest in ribosomal protein moonlighting roles has positioned RPS21 as a candidate for exploring connections between translation machinery and cellular stress signaling. Its compact size (~15 kDa) and solubility make it experimentally tractable for biochemical assays and interaction studies.
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