| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPP40 |
| Uniprot No | O75818 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363 aa |
| 活性数据 | MATLRRLREA PRHLLVCEKS NFGNHKSRHR HLVQTHYYNY RVSFLIPECG ILSEELKNLV MNTGPYYFVK NLPLHELITP EFISTFIKKG SCYALTYNTH IDEDNTVALL PNGKLILSLD KDTYEETGLQ GHPSQFSGRK IMKFIVSIDL MELSLNLDSK KYERISWSFK EKKPLKFDFL LAWHKTGSEE STMMSYFSKY QIQEHQPKVA LSTLRDLQCP VLQSSELEGT PEVSCRALEL FDWLGAVFSN VDLNNEPNNF ISTYCCPEPS TVVAKAYLCT ITGFILPEKI CLLLEHLCHY FDEPKLAPWV TLSVQGFADS PVSWEKNEHG FRKGGEHLYN FVIFNNQDYW LQMAVGANDH CPP |
| 分子量 | 41.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人RPP40蛋白的模拟参考文献示例(注：部分信息为合理推测，实际文献需以数据库检索为准)：
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1. **文献名称**: "Cloning and Expression of Human RNase P Protein p40 in Escherichia coli"
**作者**: Hannan, L., et al.
**摘要**: 该研究首次报道了人源RPP40(RNase P亚基p40)的基因克隆及在原核系统中的重组表达。作者通过大肠杆菌表达系统获得可溶性蛋白，并验证其与RNase P RNA组分的相互作用能力，为后续酶活性分析奠定了基础。
2. **文献名称**: "Structural Insights into RPP40’s Role in tRNA Processing by Cryo-EM"
**作者**: Xiao, S., et al.
**摘要**: 利用冷冻电镜技术解析了重组人RPP40蛋白在RNase P复合体中的三维结构，揭示了其通过特定结构域与RNA及其他蛋白亚基(如RPP30)结合，调控tRNA前体5'端加工的分子机制。
3. **文献名称**: "Functional Characterization of Recombinant RPP40 in Mitochondrial RNase P Complex"
**作者**: Lopez, M.D., et al.
**摘要**: 研究通过真核表达系统纯化重组RPP40蛋白，证明其在线粒体RNase P复合体中的必要性，并通过敲除实验表明RPP40缺失会导致tRNA加工缺陷及细胞能量代谢异常。
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提示：以上文献为示例性质，实际研究中建议通过PubMed或Google Scholar检索关键词(如“human RPP40 recombinant”、“RNase P subunit p40”)获取真实文献。
**Background of Recombinant Human RPP40 Protein**
Recombinant human RPP40 (Ribonuclease P Protein subunit p40) is a key component of RNase P, a conserved ribonucleoprotein complex essential for processing precursor transfer RNA (tRNA). RNase P catalyzes the 5' end maturation of tRNAs, a critical step in RNA metabolism and protein synthesis. RPP40. along with other protein subunits (e.g., RPP30. RPP38), associates with the catalytic RNA subunit (H1 RNA in humans) to form the functional holoenzyme. Structurally, RPP40 contains an Alu domain, a feature shared with proteins involved in RNA recognition and processing.
As a recombinant protein, RPP40 is produced via genetic engineering, often in *E. coli* or mammalian expression systems, enabling high-purity yields for research. Studies on recombinant RPP40 focus on elucidating its role in RNase P assembly, substrate binding, and enzymatic activity. Dysregulation of RNase P has been linked to diseases, including cancers and neurological disorders, positioning RPP40 as a potential biomarker or therapeutic target. Additionally, recombinant RPP40 facilitates *in vitro* studies on tRNA processing mechanisms and interactions with other RNase P subunits. Its applications extend to structural biology (e.g., crystallography) and exploring evolutionary conservation of RNA-protein complexes. Research on RPP40 contributes to understanding fundamental RNA-processing pathways and their implications in human health.
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