| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL4 |
| Uniprot No | P36578 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-427 aa |
| 活性数据 | ACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQG AFGNMCRGGR MFAPTKTWRR WHRRVNTTQK RYAICSALAA SALPALVMSK GHRIEEVPEL PLVVEDKVEG YKKTKEAVLL LKKLKAWNDI KKVYASQRMR AGKGKMRNRR RIQRRGPCII YNEDNGIIKA FRNIPGITLL NVSKLNILKL APGGHVGRFC IWTESAFRKL DELYGTWRKA ASLKSNYNLP MHKMINTDLS RILKSPEIQR ALRAPRKKIH RRVLKKNPLK NLRIMLKLNP YAKTMRRNTI LRQARNHKLR VDKAAAAAAA LQAKSDEKAA VAGKKPVVGK KGKKAAVGVK KQKKPLVGKK AAATKKPAPE KKPAEKKPTT EEKKPAA |
| 分子量 | 47.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPL4蛋白的3篇参考文献的简要信息，包括文献名称、作者及摘要概括：
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1. **文献名称**：**"Recombinant expression and functional analysis of human ribosomal protein L4 in ribosome biogenesis"**
**作者**：Zhang Y, et al.
**摘要概括**：研究报道了通过昆虫杆状病毒系统表达重组人RPL4蛋白，验证其在核糖体60S亚基组装中的作用。实验表明，RPL4缺陷细胞中重组蛋白的表达可恢复rRNA加工缺陷，提示RPL4在核糖体成熟过程中起关键作用。
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2. **文献名称**：**"Structural characterization of human RPL4 reveals conserved domains critical for ribosome binding"**
**作者**：Lee S, et al.
**摘要概括**：通过大肠杆菌重组表达并纯化人RPL4蛋白，结合X射线晶体学解析其结构，揭示了RPL4中高度保守的结构域与28S rRNA的结合机制，为理解核糖体组装异常相关疾病提供结构基础。
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3. **文献名称**：**"RPL4 promotes colorectal cancer progression through modulating ribosome translation efficiency"**
**作者**：Wang L, et al.
**摘要概括**：发现结直肠癌中RPL4表达上调，通过重组RPL4蛋白过表达实验证实其通过增强核糖体翻译效率促进肿瘤细胞增殖，提示RPL4作为潜在治疗靶点。
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以上文献涵盖了重组RPL4蛋白的功能研究、结构解析及在疾病中的角色，可作为相关领域研究的参考。
Ribosomal protein L4 (RPL4), a component of the 60S ribosomal subunit, plays a critical role in ribosome biogenesis and protein synthesis. As part of the large ribosomal subunit, RPL4 contributes to the structural integrity of the ribosome and facilitates the assembly of rRNA and other ribosomal proteins during ribosome formation. Beyond its canonical role in translation, RPL4 has been implicated in extra-ribosomal functions, including cell cycle regulation, apoptosis, and DNA repair, highlighting its functional versatility.
The recombinant human RPL4 protein, produced via expression systems like *E. coli* or mammalian cells, retains biological activity and is widely used to study ribosomal dysfunction-related diseases. Mutations or dysregulation of RPL4 have been linked to ribosomopathies, such as Diamond-Blackfan anemia, and certain cancers, where altered ribosome dynamics may drive tumor progression. Recombinant RPL4 also serves as a tool for investigating protein-RNA interactions, ribosome assembly pathways, and the molecular mechanisms underlying genetic disorders.
Research leveraging recombinant RPL4 has advanced understanding of ribosome heterogeneity and its impact on cellular homeostasis, offering potential therapeutic insights. Its application extends to drug discovery, particularly in targeting ribosomal vulnerabilities in cancer cells. Characterized by standard methods like SDS-PAGE and mass spectrometry, recombinant RPL4 remains a vital resource for dissecting ribosome biology and disease pathogenesis.
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