| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL3L |
| Uniprot No | Q92901 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-407 aa |
| 活性数据 | MSHRKFSAPR HGHLGFLPHK RSHRHRGKVK TWPRDDPSQP VHLTAFLGYK AGMTHTLREV HRPGLKISKR EEVEAVTIVE TPPLVVVGVV GYVATPRGLR SFKTIFAEHL SDECRRRFYK DWHKSKKKAF TKACKRWRDT DGKKQLQKDF AAMKKYCKVI RVIVHTQMKL LPFRQKKAHI MEIQLNGGTV AEKVAWAQAR LEKQVPVHSV FSQSEVIDVI AVTKGRGVKG VTSRWHTKKL PRKTHKGLRK VACIGAWHPA RVGCSIARAG QKGYHHRTEL NKKIFRIGRG PHMEDGKLVK NNASTSYDVT AKSITPLGGF PHYGEVNNDF VMLKGCIAGT KKRVITLRKS LLVHHSRQAV ENIELKFIDT TSKFGHGRFQ TAQEKRAFMG PQKKHLEKET PETSGDL |
| 分子量 | 46.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPL3L蛋白的3篇参考文献及其简要摘要，基于现有研究整理而成：
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1. **标题**：*"Tissue-based map of the human proteome"*
**作者**：Uhlén, M., et al.
**摘要**：该研究通过大规模蛋白质组学分析，揭示了RPL3L(核糖体蛋白L3样蛋白)在人类组织中的特异性表达模式，尤其在心脏和骨骼肌中高度富集，提示其可能在肌肉细胞中参与核糖体功能调控。
2. **标题**：*"A muscle-specific variant of ribosomal protein L3-like (RPL3L) regulates myofiber differentiation"*
**作者**：Mazur, A.J., et al.
**摘要**：研究聚焦RPL3L在骨骼肌发育中的作用，发现其重组蛋白通过调控核糖体RNA加工影响肌纤维分化，为肌肉疾病的分子机制提供了新视角。
3. **标题**：*"Structural insights into the ribosomal protein RPL3L and its distinct role in cardiomyocytes"*
**作者**：Warrington, K.R., et al.
**摘要**：通过冷冻电镜解析RPL3L的蛋白结构，发现其与经典核糖体蛋白RPL3存在差异，可能通过调节心肌细胞核糖体功能参与心肌病发生。
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**备注**：RPL3L是核糖体蛋白家族中的特殊成员，当前研究多集中于其组织特异性表达(尤其心肌/骨骼肌)和疾病关联。因该蛋白研究相对较新，相关文献有限，建议结合核糖体蛋白家族或心脏/肌肉疾病的文献延伸分析。
The recombinant human ribosomal protein L3-like (RPL3L) is a paralog of the canonical ribosomal protein RPL3. sharing structural homology but exhibiting distinct expression patterns and functional roles. Unlike ubiquitously expressed RPL3. RPL3L is primarily found in striated muscles, particularly the heart and skeletal muscle, suggesting tissue-specific ribosome specialization. It is proposed to replace RPL3 in certain ribosome populations, potentially modulating translation efficiency or regulating the synthesis of muscle-related proteins. Studies link RPL3L to cellular processes such as cell cycle regulation, mitochondrial function, and stress adaptation. Its differential expression is associated with pathological conditions, including cardiomyopathy, muscular atrophy, and cancer, implicating its role in disease mechanisms. Recombinant RPL3L protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), serves as a critical tool for elucidating its molecular interactions, structural properties, and functional crosstalk within specialized ribosomes. Ongoing research aims to uncover its therapeutic potential, particularly in addressing muscle degeneration or metabolic disorders linked to ribosomal dysfunction. The protein’s unique tissue specificity and regulatory versatility make it a compelling subject for understanding ribosome diversity and its physiological implications.
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