| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL36A |
| Uniprot No | P83881 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-106 aa |
| 活性数据 | MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCKH FELGGDKKRK GQVIQF |
| 分子量 | 12.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇假设性的关于重组人RPL36A蛋白的参考文献(实际文献可能需要通过学术数据库查询)：
1. **《Structural and Functional Analysis of Human RPL36A in Ribosome Assembly》**
- **作者**: Zhang, L., et al.
- **摘要**: 研究通过X射线晶体学解析了重组人RPL36A的原子结构，揭示其在核糖体小亚基组装中的关键作用，并发现该蛋白与18S rRNA的特定区域相互作用，可能影响翻译起始效率。
2. **《RPL36A as a Novel Regulator of p53-dependent Apoptosis in Cancer Cells》**
- **作者**: Gupta, S., & Wang, H.
- **摘要**: 发现重组人RPL36A在癌症中过表达，并通过调控p53稳定性参与细胞凋亡途径。敲低RPL36A显著抑制肿瘤生长，提示其作为潜在治疗靶点。
3. **《Recombinant Expression and Purification of Human RPL36A in E. coli》**
- **作者**: Müller, R., et al.
- **摘要**: 报道了一种高效制备重组人RPL36A的方法，利用大肠杆菌表达系统优化蛋白可溶性和纯化流程，并验证了纯化蛋白在体外核糖体重建实验中的功能活性。
注：以上文献为示例，具体内容需参考真实发表的科研论文。建议通过PubMed、Web of Science等平台以“RPL36A”、“ribosomal protein L36a”等关键词检索最新研究。
**Background of Recombinant Human RPL36A Protein**
Ribosomal Protein L36a (RPL36A) is a component of the 60S large ribosomal subunit, playing a crucial role in protein synthesis by facilitating mRNA decoding and polypeptide chain elongation. As a member of the ribosome proteome, RPL36A contributes to ribosomal stability and ensures efficient translation. Structurally, it contains a conserved RNA-binding domain that interacts with 28S rRNA, critical for ribosome assembly and function.
The recombinant human RPL36A protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form. This enables studies on its molecular interactions, structural roles, and dysregulation in diseases. Aberrant RPL36A expression has been linked to cancers, developmental disorders, and viral infection responses, suggesting its potential as a diagnostic marker or therapeutic target. Recombinant variants, often tagged for purification (e.g., His-tag), are vital for *in vitro* assays, structural analyses (e.g., cryo-EM), and exploring ribosome biogenesis mechanisms. Research continues to uncover its extraribosomal roles in cellular stress responses and apoptosis regulation.
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