| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL27A |
| Uniprot No | P46776 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-148 aa |
| 活性数据 | PSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGLHH HRINFDKYHP GYFGKVGMKH YHLKRNQSFC PTVNLDKLWT LVSEQTRVNA AKNKTGAAPI IDVVRSGYYK VLGKGKLPKQ PVIVKAKFFS RRAEEKIKSV GGACVLVA |
| 分子量 | 16.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPL27A蛋白的3篇参考文献摘要及作者信息：
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1. **文献名称**：*Expression and purification of recombinant human RPL27A in Escherichia coli for structural studies*
**作者**：Li, X. et al.
**摘要**：报道了通过原核表达系统高效表达并纯化重组人RPL27A蛋白的方法，利用His标签亲和层析技术获得高纯度蛋白，为后续晶体结构解析及功能研究奠定基础。
2. **文献名称**：*RPL27A regulates cell proliferation and apoptosis via the MDM2-p53 signaling pathway in colorectal cancer*
**作者**：Wang, Y. et al.
**摘要**：研究发现重组人RPL27A在结直肠癌细胞中过表达可通过结合MDM2调控p53稳定性，影响肿瘤细胞增殖和凋亡，提示RPL27A在癌症治疗中的潜在靶点价值。
3. **文献名称**：*Ribosomal protein L27A (RPL27A) facilitates 60S subunit assembly and interacts with specific rRNA domains*
**作者**：Gamalinda, M. et al.
**摘要**：通过酵母和重组人RPL27A蛋白的交叉研究，揭示了该蛋白在核糖体大亚基组装中的关键作用，并阐明了其与28S rRNA特定区域的相互作用机制。
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注：以上文献为示例性质，实际引用时请核实具体论文数据库(如PubMed、Web of Science)中的真实文献信息。
Recombinant human RPL27A (Ribosomal Protein L27a) is a small, evolutionarily conserved protein component of the 60S subunit of eukaryotic ribosomes. As part of the ribosomal machinery, it plays a fundamental role in protein biosynthesis by facilitating mRNA decoding and peptide bond formation. RPL27A is encoded by the RPL27A gene located on chromosome 11 in humans. Beyond its canonical role in translation, emerging studies suggest its involvement in non-ribosomal functions, including cell cycle regulation, apoptosis, and interactions with oncogenic pathways.
The recombinant form of RPL27A is typically produced using bacterial (e.g., *E. coli*) or eukaryotic expression systems, enabling detailed structural and functional analyses. Its recombinant expression allows for applications in crystallography, protein-protein interaction studies, and high-throughput drug screening, particularly in cancer research where dysregulated ribosome biogenesis is linked to tumorigenesis. Mutations or aberrant expression of RPL27A have been associated with certain cancers, making it a potential biomarker or therapeutic target.
Despite its small size (~15 kDa), RPL27A's conserved residues and interactions with rRNA or ribosomal proteins highlight its structural importance in ribosome assembly. Research continues to explore its extra-ribosomal roles and disease relevance, underscoring its dual utility in basic biology and translational medicine.
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