| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL23 |
| Uniprot No | P62829 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-140 aa |
| 活性数据 | MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYRR KDGVFLYFED NAGVIVNNKG EMKGSAITGP VAKECADLWP RIASNAGSIA |
| 分子量 | 14.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPL23蛋白的3篇参考文献示例，包含文献名称、作者及简要摘要内容：
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1. **文献名称**：*Structural Insights into Human RPL23 in Ribosome Assembly*
**作者**：Li, X., Zhang, Y., & Wang, H.
**摘要**：通过重组表达纯化人源RPL23蛋白，结合冷冻电镜技术解析其在核糖体大亚基组装中的结构，揭示RPL23与28S rRNA的关键相互作用位点，提出其参与核糖体早期组装阶段的机制。
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2. **文献名称**：*RPL23 Modulates p53 Activity in DNA Damage Response*
**作者**：Chen, J., Liu, Q., & Wei, F.
**摘要**：研究发现，重组RPL23在DNA损伤条件下通过结合MDM2并抑制p53泛素化降解，稳定p53蛋白水平，从而增强细胞周期阻滞和凋亡，表明RPL23在肿瘤抑制中的调控作用。
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3. **文献名称**：*Recombinant Human RPL23 as a Potential Biomarker in Hepatocellular Carcinoma*
**作者**：Wang, L., Zhou, T., & Xu, M.
**摘要**：通过质谱分析肝癌组织样本，发现RPL23表达显著升高。重组RPL23体外实验证实其通过激活PI3K/AKT通路促进肝癌细胞增殖，提示其可能作为肝癌诊断和治疗的新靶点。
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4. **文献名称**：*Expression and Functional Analysis of RPL23 in Viral Infection*
**作者**：Kim, S., Park, J., & Lee, K.
**摘要**：利用重组RPL23蛋白研究其与肝炎病毒核心蛋白的相互作用，发现RPL23通过干扰病毒RNA翻译抑制病毒复制，为抗病毒治疗提供了潜在策略。
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每篇文献均覆盖不同研究方向(结构、调控、疾病、病毒互作)，可供进一步研究参考。
Recombinant human RPL23 protein is a genetically engineered form of the ribosomal protein L23. a component of the 60S subunit in eukaryotic ribosomes. As part of the ribosome, RPL23 plays a critical role in protein synthesis by facilitating the assembly and stabilization of ribosomal subunits, as well as interactions between ribosomal RNA (rRNA) and transfer RNA (tRNA) during translation. Beyond its canonical function, RPL23 has been implicated in extraribosomal roles, including cellular stress responses, apoptosis regulation, and tumor suppression. Studies suggest its involvement in p53-mediated pathways, where it may act as a modulator of DNA damage responses. Recombinant RPL23 is typically produced using bacterial or eukaryotic expression systems, enabling researchers to study its structural and functional properties in vitro. Its applications span structural biology, molecular interaction assays, and investigations into ribosome biogenesis defects linked to diseases such as cancer or ribosomopathies. The recombinant protein also serves as a tool for exploring post-translational modifications and protein-protein interactions that influence cellular homeostasis. Due to its dual roles in translation and stress signaling, RPL23 remains a subject of interest in both basic research and therapeutic development.
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