| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPIP8 |
| Uniprot No | Q59EK9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446 aa |
| 活性数据 | MEASFVQTTM ALGLSSKKAS SRNVAVERKN LITVCRFSVK TLLEKYTAEP IDDSSEEFVN FAAILEQILS HRFKACAPAG PVSWFSSDGQ RGFWDYIRLA CSKVPNNCVS SIENMENIST ARAKGRAWIR VALMEKRMSE YITTALRDTR TTRRFYDSGA IMLRDEATIL TGMLIGLSAI DFSFCLKGEV LDGKTPVVID YTPYLKFTQS YDYLTDEEER HSAESSTSED NSPEHPYLPL VTDEDSWYSK WHKMEQKFRI VYAQKGYLEE LVRLRESQLK DLEAENRRLQ LQLEEAAAQN QREKRELEGV ILELQEQLTG LIPSDHAPLA QGSKELTTPL VNQWPSLGTL NGAEGASNSK LYRRHSFMST EPLSAEASLS SDSQRLGEGT RDEEPWGPIG KDPTPSMLGL CGSLASIPSC KSLASFKSNE CLVSDSPEGS PALSPS |
| 分子量 | 49.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为虚构示例，仅供参考的3-4条关于重组人RPIP8蛋白的文献：
1. **文献名称**：*Role of RPIP8 in NF-κB Signaling and Apoptosis Regulation*
**作者**：Chen L, et al.
**摘要**：研究发现RPIP8通过结合TRAF2蛋白调控NF-κB信号通路，抑制肿瘤细胞凋亡，可能成为癌症治疗靶点。
2. **文献名称**：*Structural Characterization of RPIP8 and Its Interaction with MYD88*
**作者**：Wang X, et al.
**摘要**：通过晶体学解析RPIP8蛋白结构，揭示其N端结构域与MYD88的关键互作位点，提示其在先天免疫中的作用。
3. **文献名称**：*RPIP8 Deficiency Alters Inflammatory Response in Mouse Models*
**作者**：Zhang Y, et al.
**摘要**：敲除RPIP8的小鼠显示TLR信号通路异常激活，导致过度炎症反应，表明其负向调节免疫的机制。
4. **文献名称**：*RPIP8 Overexpression in Neurodegenerative Disorders*
**作者**：Kim S, et al.
**摘要**：在阿尔茨海默病模型中，RPIP8的过表达通过减少Aβ蛋白沉积改善神经元存活，提示其潜在治疗价值。
**注意**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等平台用关键词(如“RPIP8”、“Recombinant human RPIP8”)检索获取。
Recombinant human RPIP8 (Rap2-interacting protein 8), also known as RUNDC3B, is a protein encoded by the *RUNDC3B* gene located on chromosome 7p22.1. Originally identified as a Rap2 GTPase-interacting partner, RPIP8 plays roles in intracellular signaling pathways regulating cytoskeletal dynamics, cell adhesion, and vesicular trafficking. Structurally, it contains conserved RUN and SH3 domain-binding motifs, facilitating protein-protein interactions critical for its function. RPIP8 is ubiquitously expressed but shows higher levels in the brain, suggesting a potential role in neuronal processes. Studies link it to mTOR signaling modulation and autophagy regulation, with implications in cancer progression, neuronal development, and immune responses.
The recombinant form of RPIP8 is typically produced via *E. coli* or mammalian expression systems, enabling biochemical and functional studies. Its recombinant expression aids in elucidating interactions with signaling molecules like Rap2. TSC2. and components of the mTORC1 complex. Research highlights RPIP8's dual role in cancer: as a tumor suppressor in gliomas by inhibiting cell proliferation, and as a metastasis promoter in breast cancer via EMT activation. Dysregulation of RPIP8 has also been observed in neurodevelopmental disorders and inflammatory diseases. Despite progress, its precise molecular mechanisms and tissue-specific functions remain under active investigation, making it a target for therapeutic exploration in multiple pathologies.
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