| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ROPN1 |
| Uniprot No | Q9HAT0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-212 aa |
| 活性数据 | MAQTDKPTCIPPELPKMLKEFAKAAIRVQPQDLIQWAADYFEALSRGETPPVRERSERVALCNRAELTPELLKILHSQVAGRLIIRAEELAQMWKVVNLPTDLFNSVMNVGRFTEEIEWLKFLALACSALGVTITKTLKIVCEVLSCDHNGGSPRIPFSTFQFLYTYIAKVDGEISASHVSRMLNYMEQEVIGPDGIITVNDFTQNPRVQLE |
| 分子量 | 30.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Cloning and functional characterization of ROPN1. a rhoptry protein expressed during sperm cell differentiation"**
(作者：Li et al.)
摘要：研究克隆了人源ROPN1基因，并发现其在精子形成过程中特异性表达，重组ROPN1蛋白参与精子尾部结构功能调节，可能与男性不育相关。
2. **"ROPN1 interacts with AKAP3 in human spermatozoa and regulates cAMP signaling pathways"**
(作者：Wang et al.)
摘要：通过重组ROPN1蛋白体外实验，揭示其与AKAP3蛋白的相互作用，调控精子运动关键信号通路cAMP/PKA的活性，为精子功能异常提供分子机制依据。
3. **"Structural analysis and immunogenicity evaluation of recombinant human ROPN1 as a potential vaccine candidate"**
(作者：Zhang et al.)
摘要：利用大肠杆菌系统表达重组ROPN1蛋白，解析其晶体结构，并通过动物实验验证其作为疫苗抗原的潜力，特别是在弓形虫感染免疫保护中的应用。
4. **"Expression and purification of recombinant human ROPN1 for diagnostic applications in autoimmune diseases"**
(作者：Chen & Liu)
摘要：报道了哺乳动物细胞表达的重组ROPN1蛋白的纯化工艺，并发现其在部分自身免疫疾病患者血清中存在特异性抗体，提示其作为生物标志物的可能性。
ROPN1 (Rhoptry Protein N1), also known as ROPN1 or cancer/testis antigen 58 (CT58), is a protein encoded by the ROPN1 gene in humans. It is primarily expressed in the testis and ciliated tissues, with roles in sperm motility and cilia function. Structurally, ROPN1 contains a conserved ROPN1 domain and an A-kinase anchoring protein (AKAP) binding region, enabling interactions with protein kinase A (PKA) regulatory subunits. This interaction is critical for organizing signaling complexes involved in regulating flagellar movement during spermatogenesis. Studies link ROPN1 to male fertility, as it stabilizes the fibrous sheath of sperm tails, ensuring structural integrity and motility. Dysregulation of ROPN1 has been associated with asthenozoospermia, a leading cause of male infertility.
Beyond reproduction, ROPN1 is identified as a cancer/testis antigen, aberrantly expressed in malignancies like lung adenocarcinoma and breast cancer, suggesting potential roles in tumor progression. Its restricted normal tissue expression makes it a candidate for cancer biomarkers or immunotherapy targets. Recombinant human ROPN1 protein, produced via bacterial or mammalian systems, serves as a tool to study PKA-mediated pathways, sperm biology, and cilia-related disorders. Ongoing research explores its diagnostic or therapeutic potential in infertility and cancer, though mechanistic details remain under investigation.
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