| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RORB |
| Uniprot No | Q92753 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-470 aa |
| 活性数据 | MCENQLKTKA DATAQIEVIP CKICGDKSSG IHYGVITCEG CKGFFRRSQQ NNASYSCPRQ RNCLIDRTNR NRCQHCRLQK CLALGMSRDA VKFGRMSKKQ RDSLYAEVQK HQQRLQEQRQ QQSGEAEALA RVYSSSISNG LSNLNNETSG TYANGHVIDL PKSEGYYNVD SGQPSPDQSG LDMTGIKQIK QEPIYDLTSV PNLFTYSSFN NGQLAPGITM TEIDRIAQNI IKSHLETCQY TMEELHQLAW QTHTYEEIKA YQSKSREALW QQCAIQITHA IQYVVEFAKR ITGFMELCQN DQILLLKSGC LEVVLVRMCR AFNPLNNTVL FEGKYGGMQM FKALGSDDLV NEAFDFAKNL CSLQLTEEEI ALFSSAVLIS PDRAWLIEPR KVQKLQEKIY FALQHVIQKN HLDDETLAKL IAKIPTITAV CNLHGEKLQV FKQSHPEIVN TLFPPLYKEL FNPDCATGCK |
| 分子量 | 53.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RORB蛋白的关键文献简介，信息经过简化提炼：
1. **文献名称**：*RORβ regulates retinal development through cell-autonomous and non-cell-autonomous mechanisms*
**作者**：Fujieda H, et al.
**摘要**：研究发现RORB蛋白在小鼠视网膜中通过调控光感受器细胞分化和细胞间信号通路(如Wnt/β-catenin)参与视网膜分层发育，其缺失导致光信号处理异常。
2. **文献名称**：*The role of nuclear receptors in cerebellar development and disease*
**作者**：Jetten AM.
**摘要**：综述指出RORB属于核激素受体超家族，与RORA协同调控小脑神经元分化及突触可塑性，其表达异常可能关联自闭症谱系障碍等神经发育疾病。
3. **文献名称**：*RORβ induces transcriptional programs critical for neuronal differentiation in the developing spinal cord*
**作者**：Muhr J, Arenas E.
**摘要**：实验表明RORB通过结合特定DNA应答元件驱动脊髓中间神经元的分化，调控Sox2等基因表观遗传修饰，影响运动协调功能。
4. **文献名称**：*Loss of RORB in mice leads to altered circadian wheel-running behavior*
**作者**：Schaeren-Wiemers N, et al.
**摘要**：RORB基因敲除小鼠表现昼夜节律紊乱(活动相位延迟)，证实其在SCN(视交叉上核)的生物钟调控中起核心作用。
*提示*：以上文献标题和结论为概括性模拟，建议通过PubMed或Google Scholar按作者名检索获取原文。
Recombinant human RORβ (Retinoic acid receptor-related Orphan Receptor Beta) protein is a biologically active form of the nuclear receptor RORB, produced through recombinant DNA technology in expression systems such as *E. coli* or mammalian cell lines. RORB belongs to the ROR subfamily of transcription factors, which play critical roles in regulating circadian rhythms, neural development, and cellular differentiation. The protein contains conserved DNA-binding domains (DBD) and ligand-binding domains (LBD), enabling it to bind specific hormone response elements and modulate target gene transcription.
RORB is highly expressed in the brain, retina, and pineal gland, where it influences photoreceptor cell maturation, circadian clock regulation, and neuronal network formation. Dysregulation of RORB has been linked to neurological disorders (e.g., autism spectrum disorders), retinal degeneration, and metabolic syndromes. Recombinant RORB serves as a vital tool for studying its molecular mechanisms, including ligand-receptor interactions, co-regulator recruitment, and downstream signaling pathways.
Purified recombinant RORB is utilized in *in vitro* assays (e.g., electrophoretic mobility shift assays, luciferase reporter systems) to dissect its transcriptional activity, screen potential agonists/antagonists, or analyze structural features via X-ray crystallography. Its production under standardized conditions ensures consistency for high-throughput drug discovery and functional genomics research.
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