| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL10L |
| Uniprot No | Q96L21 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-214 aa |
| 活性数据 | MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLGG HMVSDEYEQL SSEALEAARI CANKYMVKSC GRDGFHMRVR LHPFHVIRIN KMLSCAGADR LQTGMRGAFG KPQGTVARVH IGQVIMSIRT KLQNEEHVIE ALRRAKFKFP GRQKIHISKK WGFTKFNADE FEDMVAKKCL IPDGCGVKYV PSHGPLDKWR VLHS |
| 分子量 | 24.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPL10L蛋白的3-4篇文献示例，内容基于模拟研究背景整理：
1. **文献名称**：*RPL10L is essential for male fertility by regulating spermatogenesis in mice*
**作者**：Zhang Y. et al.
**摘要**：研究揭示了RPL10L在小鼠精子发生中的关键作用，其缺失导致生殖细胞减数分裂异常和精子形成缺陷，提示RPL10L通过与核糖体复合物互作维持生殖细胞发育。
2. **文献名称**：*Expression and oncogenic role of RPL10L in hepatocellular carcinoma*
**作者**：Wang X. et al.
**摘要**：发现RPL10L在肝癌细胞中异常高表达，促进细胞周期进程和增殖，机制可能与调控p53信号通路相关，提示其作为癌症治疗靶点的潜力。
3. **文献名称**：*Structural characterization of recombinant human RPL10L protein*
**作者**：Li H. et al.
**摘要**：报道利用原核表达系统成功获得高纯度重组人RPL10L蛋白，并通过X射线晶体学解析其三维结构，为后续功能研究提供分子基础。
4. **文献名称**：*Conservation and divergence of RPL10L in mammalian germline development*
**作者**：Smith J. et al.
**摘要**：跨物种比较发现RPL10L在生殖细胞中高度保守，尤其在减数分裂阶段的特异性表达模式，提示其在进化中维持生殖功能的稳定性。
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**注意**：上述文献为示例，实际引用时需通过学术数据库验证信息准确性。建议使用PubMed、Web of Science等工具搜索最新相关研究。
RPL10L (Ribosomal Protein L10-like) is a member of the ribosomal protein family, playing a role in ribosome assembly and protein synthesis. It shares significant homology with RPL10. a core component of the 60S ribosomal subunit, but exhibits distinct expression patterns and functional divergence. Unlike ubiquitously expressed RPL10. RPL10L is predominantly expressed in germ cells, particularly during spermatogenesis, suggesting a specialized role in male reproductive biology. Studies indicate that RPL10L may compensate for RPL10 loss in germ cells, maintaining ribosome stability and translational fidelity critical for gamete development.
The human *RPL10L* gene, located on the X chromosome (Xq22.3), is evolutionarily conserved but absent in certain species, highlighting its lineage-specific adaptation. Epigenetic regulation and alternative splicing further modulate its tissue-specific activity. While its precise molecular mechanisms remain under investigation, RPL10L has been implicated in male infertility due to its association with sperm maturation defects in model organisms. Emerging evidence also links dysregulated RPL10L expression to certain cancers, though its oncogenic or tumor-suppressive role remains controversial.
Research on RPL10L underscores the functional diversification of ribosomal proteins beyond canonical translation, emphasizing cell type- and context-dependent roles in development and disease. Its study provides insights into ribosome heterogeneity and reproductive biology.
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