| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL21 |
| Uniprot No | P46778 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-160 aa |
| 活性数据 | TNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRIN VRIEHIKHSK SRDSFLKRVK ENDQKKKEAK EKGTWVQLKR QPAPPREAHF VRTNGKEPEL LEPIPYEFMA |
| 分子量 | 18.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPL21蛋白的3篇参考文献及其摘要内容概要：
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1. **标题**：*Cloning and expression of human ribosomal protein L21: characterization of a structural-functional homolog in carcinogenesis*
**作者**：Chen X, et al.
**摘要**：该研究克隆并表达了人源RPL21重组蛋白，通过原核系统纯化后分析其理化性质。发现RPL21在多种癌细胞系中表达异常，体外实验表明其过表达可能通过调控核糖体组装促进肿瘤细胞增殖。
2. **标题**：*Recombinant RPL21 interacts with MDM2 and modulates p53 stability in colorectal cancer cells*
**作者**：Wang L, et al.
**摘要**：研究利用重组人RPL21蛋白进行免疫共沉淀及质谱分析，揭示其与肿瘤抑制蛋白p53的调控因子MDM2直接结合，并影响p53的泛素化降解途径，提示RPL21可能在结直肠癌发展中具有非核糖体功能。
3. **标题**：*Structural insights into human RPL21 folding and ribosome assembly defects*
**作者**：Zhang Y, et al.
**摘要**：通过X射线晶体学解析重组RPL21的三维结构，发现其N端结构域对核糖体大亚基的组装至关重要。功能实验表明，特定突变会破坏核糖体装配，导致细胞周期阻滞。
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注：上述文献为示例，实际引用时需核实具体来源及发表信息。建议通过PubMed或Web of Science等平台以“recombinant RPL21 human”为关键词检索最新研究。
Ribosomal protein L21 (RPL21) is a component of the 60S large ribosomal subunit in eukaryotes, playing a critical role in protein biosynthesis. As part of the ribosome, RPL21 contributes to the structural stability and functional dynamics of the ribosomal complex during mRNA translation. It belongs to the L21e family of ribosomal proteins and is highly conserved across species, underscoring its essential role in cellular viability. In humans, the RPL21 gene is located on chromosome 13 and encodes a basic protein rich in arginine and lysine residues, which facilitates interactions with rRNA and other ribosomal components.
Recombinant human RPL21 protein is produced through heterologous expression systems (e.g., *E. coli* or mammalian cells) for research applications. This engineered protein retains native biochemical properties, enabling studies on ribosome assembly, translational regulation, and ribosomal dysfunction linked to diseases. Mutations or dysregulation of RPL21 have been implicated in developmental disorders, cancer progression, and neurodegenerative conditions, making it a target for mechanistic and therapeutic investigations. Recombinant RPL21 also serves as a tool for structural analysis, antibody development, and screening compounds targeting ribosome-related pathways. Its availability in purified form supports advances in understanding ribosome biology and pathologies associated with translational defects.
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