| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL37 |
| Uniprot No | P61927 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-97 aa |
| 活性数据 | TKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRAA VAASSSS |
| 分子量 | 11.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPL37蛋白的3篇参考文献的简要总结：
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1. **文献名称**：**Structural insights into the human ribosomal protein RPL37 through recombinant expression and crystallography**
**作者**：Zhang Y, et al.
**摘要**：本研究通过重组技术在大肠杆菌中表达并纯化了人源RPL37蛋白，利用X射线晶体学解析其三维结构。结果显示，RPL37的α螺旋结构域在核糖体大亚基组装中可能参与RNA结合，为研究其功能机制提供了结构基础。
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2. **文献名称**：**RPL37 modulates p53 activity by regulating ribosomal stress responses in colorectal cancer**
**作者**：Li J, Wang H, et al.
**摘要**：通过重组RPL37蛋白体外实验，发现其表达下调会激活p53通路，导致结直肠癌细胞凋亡。研究表明RPL37通过调控核糖体应激反应影响肿瘤进展，可能成为潜在的癌症治疗靶点。
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3. **文献名称**：**High-level expression and purification of recombinant human RPL37 for antibody production**
**作者**：Chen S, et al.
**摘要**：开发了一种高效的昆虫细胞表达系统，用于生产高纯度重组人RPL37蛋白，并基于此制备了特异性抗体。研究验证了抗体在免疫组化与Western blot中的实用性，为RPL37的临床检测提供了工具。
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**备注**：以上文献为模拟生成，实际引用时请以具体数据库(如PubMed、Web of Science)检索的论文为准。
**Background of Recombinant Human RPL37 Protein**
Ribosomal protein L37 (RPL37) is a component of the 60S large ribosomal subunit, playing a critical role in protein synthesis by facilitating ribosome assembly and translation elongation. As a member of the ribosomal protein family, RPL37 is highly conserved across eukaryotes, underscoring its essential function in cellular homeostasis. Structurally, it contains a conserved C2-C2 zinc finger domain, which may contribute to ribosome stability or interactions with rRNA.
Recombinant human RPL37 is produced using biotechnology methods, such as expression in *E. coli* or mammalian systems, enabling studies on its molecular mechanisms and interactions. Beyond its canonical role in translation, RPL37 has been implicated in extra-ribosomal functions, including cell cycle regulation and apoptosis. Dysregulation of RPL37 expression is linked to certain cancers, with studies suggesting its potential as a biomarker or therapeutic target. For instance, overexpression of RPL37 has been observed in hepatocellular carcinoma and leukemia, correlating with tumor progression.
Research on recombinant RPL37 also explores its involvement in stress responses and ribosomopathies. By providing a purified, functional form of the protein, recombinant RPL37 aids in structural studies, drug screening, and elucidating its role in diseases. Further investigations aim to unravel its interactions with other ribosomal components and non-ribosomal partners, enhancing understanding of ribosome biology and pathology.
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