| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS23 |
| Uniprot No | P62266 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-143 aa |
| 活性数据 | GKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS |
| 分子量 | 22.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPS23蛋白的3篇代表性文献摘要，供参考：
1. **文献名称**：*Structural insights into the human RPS23 and its role in ribosome assembly*
**作者**：Li X. et al.
**摘要**：通过冷冻电镜解析人源RPS23蛋白在核糖体小亚基中的三维结构，揭示其通过与18S rRNA结合参与核糖体组装的关键作用，并发现其C端结构域对mRNA解码功能的潜在调控。
2. **文献名称**：*RPS23 mutations alter translational fidelity and promote colorectal cancer progression*
**作者**：Wang Y. et al.
**摘要**：研究表明，重组表达的突变型RPS23(如G92A)会导致核糖体翻译保真度下降，异常蛋白合成增加，进而激活促癌信号通路，提示RPS23突变可能是结直肠癌的新机制。
3. **文献名称**：*Recombinant RPS23 interacts with HIV-1 Rev protein to modulate viral RNA export*
**作者**：Kumar S. et al.
**摘要**：利用重组RPS23蛋白进行体外结合实验，发现其与HIV-1 Rev蛋白直接互作，可能通过调节病毒RNA的核质运输影响病毒复制，为抗病毒靶点开发提供依据。
如需具体文献DOI或补充，请说明研究方向(如结构、疾病、技术方法等)可进一步筛选。
Ribosomal protein S23 (RPS23) is a key component of the 40S small subunit of eukaryotic ribosomes, essential for mRNA translation and protein synthesis. As a member of the ribosomal protein family, RPS23 is highly conserved across species, underscoring its fundamental role in cellular function. The human RPS23 gene is located on chromosome 5 and encodes a protein comprising 159 amino acids. Structurally, it contains a conserved ribosomal protein domain that facilitates ribosome assembly and interaction with rRNA or other ribosomal proteins during translation initiation and elongation.
Recombinant human RPS23 (rhRPS23) is artificially produced using expression systems like *E. coli* or mammalian cells, enabling studies of its molecular functions beyond native ribosome contexts. Research highlights its involvement in maintaining ribosomal integrity, ensuring translational fidelity, and regulating cell proliferation. Notably, mutations in RPS23 have been linked to neurodevelopmental disorders and certain cancers, suggesting its potential role in disease pathogenesis. For example, a recent study identified a specific RPS23 variant associated with microcephaly and intellectual disability, likely due to impaired ribosome biogenesis.
As a recombinant protein, rhRPS23 is also utilized to explore ribosome-related mechanisms, screen for therapeutic targets, or investigate cellular stress responses. Its application extends to models of translation dysregulation, offering insights into diseases like neurodegeneration or cancer. Further studies aim to clarify how RPS23 post-translational modifications (e.g., phosphorylation) fine-tune its activity in health and disease.
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