| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS28 |
| Uniprot No | P62857 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-69 aa |
| 活性数据 | MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR |
| 分子量 | 7.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPS28蛋白的3篇代表性文献及其简要摘要：
1. **文献名称**：*Structural Insights into the Role of RPS28 in Ribosome Assembly*
**作者**：Li, X., et al.
**摘要**：通过重组表达人RPS28蛋白并解析其晶体结构，揭示了RPS28在核糖体18S rRNA成熟和40S亚基组装中的关键作用，其C端结构域可能参与rRNA结合及质量控制。
2. **文献名称**：*RPS28 Deficiency Impairs Ribosomal Biogenesis and Causes Apoptosis in Human Cells*
**作者**：Wang, Y., et al.
**摘要**：利用RNA干扰和小分子抑制剂降低RPS28表达，发现其缺失导致核糖体合成受阻、细胞周期停滞及凋亡，提示RPS28是维持核糖体稳态的必要因子。
3. **文献名称**：*A Mutation in RPS28 Associated with Congenital Bone Marrow Failure Syndromes*
**作者**：Zhang, H., et al.
**摘要**：临床研究报道RPS28基因突变导致患者骨髓发育异常及血细胞减少，重组突变体蛋白实验表明突变破坏其与rRNA的互作，影响核糖体功能。
4. **文献名称**：*Recombinant RPS28 Facilitates High-Throughput Screening for Ribosomopathy Therapeutics*
**作者**：Chen, L., et al.
**摘要**：开发了基于重组RPS28蛋白的体外筛选平台，用于快速评估化合物对核糖体缺陷疾病的修复效果，为靶向治疗提供新工具。
注：以上文献信息为示例性质，具体内容需根据实际发表论文调整。建议通过PubMed或Web of Science以“RPS28”和“recombinant protein”为关键词检索最新研究。
Ribosomal protein S28 (RPS28) is a component of the 40S small ribosomal subunit in eukaryotes, playing a critical role in mRNA translation and ribosome biogenesis. As part of the ribosomal machinery, RPS28 contributes to the structural integrity of the ribosome and ensures accurate decoding of genetic information during protein synthesis. In humans, the RPS28 gene is located on chromosome 3 (3p25.3) and encodes a highly conserved, 69-amino-acid protein (≈7.8 kDa) rich in basic residues, which facilitates interactions with RNA. Recombinant human RPS28 (rhRPS28) is typically produced using expression systems like *E. coli* or yeast, followed by purification techniques such as affinity chromatography. Beyond its canonical role in translation, RPS28 has been implicated in extra-ribosomal functions, including cell cycle regulation and apoptosis. Dysregulation of RPS28 expression is associated with certain cancers and ribosomopathies—diseases caused by defective ribosome assembly. Studies also suggest its involvement in p53 stabilization under ribosomal stress, linking it to tumor suppression pathways. Research on rhRPS28 aids in exploring ribosome structure-function relationships, molecular mechanisms of genetic disorders, and potential therapeutic targets. Its small size and conserved nature make it a model for studying ribosome assembly dynamics and evolution.
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