| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS29 |
| Uniprot No | P62273 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-56 aa |
| 活性数据 | GHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD |
| 分子量 | 6.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPS29蛋白的参考文献示例(注：文献为虚构示例，仅供格式参考)：
1. **文献名称**: *Structural insights into human RPS29 and its role in ribosome assembly*
**作者**: Zhang Y., Wang L., Chen X.
**摘要**: 本研究利用大肠杆菌系统表达并纯化了重组人RPS29蛋白，通过X射线晶体学解析其三维结构，揭示了RPS29与18S rRNA的相互作用位点，为核糖体小亚基组装机制提供了新见解。
2. **文献名称**: *Recombinant RPS29 rescues erythroid defects in a Diamond-Blackfan anemia cellular model*
**作者**: Smith J.R., Brown T., Lee M.
**摘要**: 作者通过昆虫杆状病毒系统表达重组人RPS29.证明其在体外可恢复Diamond-Blackfan贫血患者来源细胞的核糖体生物合成缺陷及红系分化障碍，提示RPS29突变与该疾病的病理关联。
3. **文献名称**: *Optimization of recombinant human RPS29 expression in E. coli and functional characterization*
**作者**: Kim S., Park H., Nguyen T.
**摘要**: 研究报道了RPS29在大肠杆菌中的可溶性表达优化方案，采用镍亲和层析纯化获得高纯度蛋白，并验证其与MDM2蛋白的相互作用，暗示RPS29可能通过p53通路调控细胞凋亡。
4. **文献名称**: *RPS29 modulates hepatocellular carcinoma progression via mTOR signaling*
**作者**: Li Q., Zhou W., García A.
**摘要**: 通过体外重组RPS29蛋白功能实验，发现其过表达可抑制肝癌细胞增殖，并降低mTOR通路活性，表明RPS29在肝癌中具有肿瘤抑制潜力。
(注：以上文献为基于领域知识的假设性示例，实际文献需通过学术数据库检索确认。)
Ribosomal protein S29 (RPS29) is a key component of the 40S small ribosomal subunit in eukaryotes, playing a critical role in ribosome assembly and protein translation. As part of the ribosome, RPS29 contributes to mRNA decoding and ensures translational fidelity. The human RPS29 gene is located on chromosome 15q25.2 and encodes a highly conserved 55-amino acid protein with a molecular weight of approximately 6 kDa. Structurally, it contains a C-terminal domain involved in rRNA binding and a flexible N-terminal region.
Recombinant human RPS29 (rhRPS29) is produced using expression systems like *E. coli* or mammalian cells, often fused with affinity tags (e.g., His-tag) for purification. Its small size and stability make it suitable for structural studies, including crystallography and NMR, to explore ribosome architecture. Beyond its canonical role, RPS29 has been linked to extraribosomal functions, such as regulating apoptosis and cell proliferation. Dysregulation of RPS29 is associated with Diamond-Blackfan anemia (DBA), a rare bone marrow failure disorder, due to mutations affecting ribosomal biogenesis. Additionally, altered RPS29 expression has been observed in certain cancers, suggesting potential roles in tumorigenesis.
Research on rhRPS29 aids in understanding ribosomopathies and developing therapeutic strategies, including gene therapy or small-molecule interventions targeting ribosomal dysfunction. Its study also provides insights into evolutionary conservation of ribosomal proteins across species.
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