| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPS6KL1 |
| Uniprot No | Q9Y6S9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-549 aa |
| 活性数据 | MSLVACECLP SPGLEPEPCS RARSQAHVYL EQIRNRVALG VPDMTKRDYL VDAATQIRLA LERDVSEDYE AAFNHYQNGV DVLLRGIHVD PNKERREAVK LKITKYLRRA EEIFNCHLQR PLSSGASPSA GFSSLRLRPI RTLSSAVEQL RGCRVVGVIE KVQLVQDPAT GGTFVVKSLP RCHMVSRERL TIIPHGVPYM TKLLRYFVSE DSIFLHLEHV QGGTLWSHLL SQAHSRHSGL SSGSTQERMK AQLNPHLNLL TPARLPSGHA PGQDRIALEP PRTSPNLLLA GEAPSTRPQR EAEGEPTART STSGSSDLPK APGGHLHLQA RRAGQNSDAG PPRGLTWVPE GAGPVLGGCG RGMDQSCLSA DGAGRGCGRA TWSVREEQVK QWAAEMLVAL EALHEQGVLC RDLHPGNLLL DQAGHIRLTY FGQWSEVEPQ CCGEAVDNLY SAPEVGGISE LTEACDWWSF GSLLYELLTG MALSQSHPSG IQAHTQLQLP EWLSRPAASL LTELLQFEPT RRLGMGEGGV SKLKSHPFFS TIQWSKLVG |
| 分子量 | 60.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPS6KL1蛋白的3篇参考文献及其摘要概览：
1. **标题**：*RPS6KL1 phosphorylates histone H2B and regulates DNA damage response*
**作者**：Zhang Y, et al.
**摘要**：本研究揭示了重组人RPS6KL1蛋白作为核糖体S6激酶家族成员的新型功能，其在DNA损伤反应中通过磷酸化组蛋白H2B(Ser33位点)调控染色质重塑，进而影响细胞周期检查点激活和基因组稳定性。
2. **标题**：*Functional characterization of RPS6KL1 in the mTOR signaling pathway*
**作者**：Lee H, et al.
**摘要**：文章通过体外重组表达RPS6KL1蛋白，证明其与mTORC1信号通路相互作用，能够磷酸化下游底物S6核糖体蛋白，并参与调控细胞生长和代谢，为癌症靶向治疗提供新方向。
3. **标题**：*Crystal structure of the human RPS6KL1 kinase domain and insights into substrate recognition*
**作者**：Chen X, et al.
**摘要**：该研究解析了重组人RPS6KL1激酶结构域的晶体结构(分辨率为2.1Å)，结合突变分析与体外激酶实验，揭示了其底物结合口袋的构象变化及ATP结合的调控机制，解释了其与RPS6K家族其他成员的活性差异。
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(注：以上文献为虚拟示例，实际研究中建议通过PubMed等数据库以关键词“RPS6KL1 recombinant”搜索最新论文。)
Recombinant human RPS6KL1 (Ribosomal Protein S6 Kinase-like 1) is a protein belonging to the AGC family of serine/threonine kinases, structurally related to ribosomal S6 kinases (RSKs) and mitogen-activated protein kinases (MAPKs). It contains a conserved kinase domain and potential regulatory regions at its N- and C-termini. Though its precise biological role remains under investigation, RPS6KL1 is hypothesized to regulate cellular processes such as proliferation, survival, and stress responses, potentially through interactions with ribosomes or signaling cascades like the PI3K/AKT/mTOR pathway. Studies suggest its involvement in mRNA translation, metabolic regulation, and DNA damage repair. Dysregulation of RPS6KL1 has been linked to cancers, neurological disorders, and developmental defects, highlighting its clinical relevance. The recombinant form is typically produced in bacterial or mammalian expression systems, often fused with tags (e.g., His, GST) for purification and detection. It serves as a tool for elucidating kinase activity, substrate specificity, and signaling mechanisms, with potential applications in drug discovery targeting kinase-related diseases. Further research is needed to fully characterize its physiological substrates, upstream regulators, and tissue-specific functions.
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