| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RRAD |
| Uniprot No | P55042 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-308 aa |
| 活性数据 | MTLNGGGSGA GGSRGGGQER ERRRGSTPWG PAPPLHRRSM PVDERDLQAA LTPGALTAAA AGTGTQGPRL DWPEDSEDSL SSGGSDSDES VYKVLLLGAP GVGKSALARI FGGVEDGPEA EAAGHTYDRS IVVDGEEASL MVYDIWEQDG GRWLPGHCMA MGDAYVIVYS VTDKGSFEKA SELRVQLRRA RQTDDVPIIL VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV QALFEGVVRQ IRLRRDSKEA NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK SCHDLSVL |
| 分子量 | 33.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RRAD蛋白的3篇文献摘要(模拟数据，仅供参考)：
1. **文献名称**: "RRAD regulates insulin signaling and glucose uptake in skeletal muscle"
**作者**: Tanaka, K. et al. (2012)
**摘要**: 研究揭示了RRAD蛋白通过抑制胰岛素受体底物(IRS-1)的磷酸化，负调控骨骼肌中的胰岛素信号通路，可能参与2型糖尿病的病理机制。
2. **文献名称**: "Role of RRAD in cancer cell migration and invasion"
**作者**: Chen, L. et al. (2016)
**摘要**: 发现RRAD在乳腺癌细胞中高表达，通过激活RhoA/ROCK通路促进细胞迁移和侵袭，提示其作为潜在肿瘤治疗靶点。
3. **文献名称**: "Structural analysis of RRAD GTPase domain by X-ray crystallography"
**作者**: Smith, J.R. et al. (2019)
**摘要**: 首次解析了RRAD蛋白GTP酶结构域的晶体结构，揭示了其与GDP结合的构象特征，为功能机制研究提供了结构基础。
注：以上文献为示例，实际引用需通过学术数据库(如PubMed)验证具体信息。
Recombinant human RRAD (Ras-related Associated with Diabetes) protein is a genetically engineered form of the endogenous RRAD GTPase, belonging to the RGK (Rad, Gem, Kir) subfamily within the Ras superfamily. RRAD is encoded by the RRAD gene and is implicated in glucose metabolism regulation, insulin signaling, and cellular processes such as cytoskeletal dynamics and ion channel modulation. Originally identified for its association with type 2 diabetes, RRAD is expressed in insulin-sensitive tissues, including skeletal muscle, heart, and adipose tissue. It interacts with key signaling molecules like PI3K and AMPK, influencing glucose uptake and metabolic homeostasis. Dysregulation of RRAD has been linked to insulin resistance, cardiovascular disorders, and certain cancers.
The recombinant protein is typically produced using bacterial or mammalian expression systems, enabling studies of its structure-function relationships and therapeutic potential. Its stable, purified form facilitates biochemical assays, drug screening, and mechanistic research on diabetes-related pathways. Recent studies also explore RRAD's role in cancer metastasis and cardiomyocyte function. By mimicking or inhibiting native RRAD activity, the recombinant protein serves as a tool to decipher its physiological impact and validate targets for metabolic or cardiovascular therapies. Research continues to unravel its dual roles as a tumor suppressor or promoter, depending on cellular context, highlighting its complexity in disease pathogenesis.
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