| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPUSD2 |
| Uniprot No | Q8IZ73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-545 aa |
| 活性数据 | MWLDRRGWLR VLGHWRYDLR RPSFTRTWSG DKGPMAETVS TQVGTEGGLR ASHQQNGDAG GDAKVELSPG PPKPAGREVE PAPVGGEHPS AAAPGPGKHK KRRGATRERV VPPPKKRRTG VSFGDEHFAE TSYYFEGGLR KVRPYYFDFR TYCKGRWVGH SLLHVFSTEF RAQPLAYYEA AVRAGRLQLN EKPVQDLNIV LKDNDFLRNT VHRHEPPVTA EPIRLLAENE DVVVVDKPSS IPVHPCGRFR HNTVIFILGK EHQLKELHPL HRLDRLTSGV LMFAKTAAVS ERIHEQVRDR QLEKEYVCRV EGEFPTEEVT CKEPILVVSY KVGVCRVDPR GKPCETVFQR LSYNGQSSVV RCRPLTGRTH QIRVHLQFLG HPILNDPIYN SVAWGPSRGR GGYIPKTNEE LLRDLVAEHQ AKQSLDVLDL CEGDLSPGLT DSTAPSSELG KDDLEELAAA AQKMEEVAEA APQELDTIAL ASEKAVETDV MNQETDPLCA ECRLVRQDPL PQDLVMFLHA LRYKGPGFEY FSPMPAWAQD DWQKD |
| 分子量 | 61.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RPUSD2蛋白的3篇参考文献示例(注：RPUSD2相关研究较少，以下为模拟示例)：
1. **文献名称**：Structural Insights into Human RPUSD2 Protein by Recombinant Expression and Crystallography
**作者**：Zhang Y, et al.
**摘要**：报道了重组人RPUSD2蛋白在大肠杆菌中的表达与纯化，并通过X射线晶体学解析了其三维结构，揭示了其假尿苷合成酶催化结构域的特征。
2. **文献名称**：Functional Characterization of Recombinant RPUSD2 in RNA Modification Pathways
**作者**：Lee S, Kim J.
**摘要**：利用哺乳动物细胞系统重组表达了RPUSD2蛋白，证实其参与线粒体RNA假尿苷修饰过程，并通过敲除实验证明其调控细胞能量代谢的作用。
3. **文献名称**：RPUSD2 Interacts with Mitochondrial RNA Granules: A Study Using Recombinant Probes
**作者**：Garcia-Molina A, et al.
**摘要**：通过荧光标记的重组RPUSD2蛋白进行活细胞成像，发现其靶向线粒体RNA颗粒，并揭示了与线粒体转录复合物的相互作用网络。
**说明**：以上文献为模拟内容，真实研究需通过PubMed/Google Scholar以“RPUSD2 recombinant”等关键词检索。RPUSD2相关公开研究较少，建议结合其别名(如C3orf1)或功能关键词(RNA modification, pseudouridine synthase)进一步探索。
Recombinant human RPUSD2 (Ribosomal Pseudouridine Synthase Domain-containing protein 2) is a genetically engineered protein involved in RNA modification processes, particularly pseudouridylation, a common post-transcriptional alteration in ribosomal RNA (rRNA). RPUSD2 contains a conserved pseudouridine synthase domain, which catalyzes the isomerization of uridine to pseudouridine (Ψ), a modification critical for ribosome biogenesis, stability, and translational fidelity. This protein is implicated in regulating ribosome function, thereby influencing cellular protein synthesis and homeostasis.
Studies suggest RPUSD2's potential role in cancer and neurodegenerative diseases, as dysregulated pseudouridylation correlates with aberrant ribosome activity and cellular stress. Its recombinant form is typically produced in prokaryotic systems (e.g., *E. coli*) or eukaryotic cells to enable structural and functional studies. Recombinant RPUSD2 aids in elucidating enzymatic mechanisms, RNA-protein interactions, and its involvement in disease pathways. Researchers utilize it to explore therapeutic targets, given its association with cellular proliferation and apoptosis. Current research focuses on characterizing its substrate specificity, regulatory partners, and tissue-specific expression patterns to better understand its physiological and pathological contributions.
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